Structure of PDB 5xus Chain A

Receptor sequence

>5xusA (length=1206) Species: 1410628 (Lachnospiraceae bacterium ND2006)

HMSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAEDYKG
VKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEI
NLRKEIAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTT
AFTGFFDNRENMFSEEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDK
HEVQEIKEKILNSDYDVEDFFEGEFFNFVLTQEGIDVYNAIIGGFVTESG
EKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLSDRESLSFYGEGYTSDEE
VLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNGPAISTISK
DIFGEWNVIRDKWNAEYDDIHLVTEKYEDDRRKSFKKIGSFSLEQLQEYA
DADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVA
IMKDLLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIY
DAIRNYVTQKPYSKDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYY
LAIMDKKYAKCLQKIDKDDVNGNYEKINYKLLPGPNKMLPKVFFSKKWMA
YYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLIDFFKDSISRYPKWSNAYD
FNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVEEGKLYMFQI
YNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINK
CPKNIFKINTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQY
SLNEIINNFNGIRIKTDYHSLLDKKEKERFEARQNWTSIENIKELKAGYI
SQVVHKICELVEKYDAVIALEDLNVKVEKQVYQKFEKMLIDKLNYMVDKK
SNPCATGGALKGYQITNKFESFKSMSTQNGFIFYIPAWLTSKIDPSTGFV
NLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDYKNFSRTDADYIK
KWKLYSYGNRIRIFDWEEVCLTSAYKELFNKYGINYQQGDIRALLCEQSD
KAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNYEAQ
ENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLE
YAQTSV

3D structure

• PDB: 5xus Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1.
• Chain: A
• Resolution: 2.5 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	S14 K15 T16 R18 F19 K20 K51 N157 R158 R174 K253 L261 Q264 Y277 K278 V280 W355 R386 K390 K464 D505 R508 K520 N706 K707 S710 K712 H714 G715 T716 N718 H720 E743 R747 H759 I765 A766 N767 K768 N769 N772 K774 T777 Y781 Y784 K785 D786 R788 F789 Q793 H797 F863 N864 I868 T870 Y872 R887 E898 G902 S905 H909 V938 K953 K960	S15 K16 T17 R19 F20 K21 K52 N158 R159 R175 K254 L262 Q265 Y278 K279 V281 W356 R382 K386 K460 D501 R504 K516 N702 K703 S706 K708 H710 G711 T712 N714 H716 E739 R743 H755 I761 A762 N763 K764 N765 N768 K770 T773 Y777 Y780 K781 D782 R784 F785 Q789 H793 F859 N860 I864 T866 Y868 R883 E894 G898 S901 H905 V927 K942 K949
BS02	dna	A	N160 K167 S168 T169 G242 V245 K251 N256 E257 N260 S286 S288 Y290 K349 W355 N509 T512 Q513 K514 D535 D537 K538 Y542 L585 P587 K595 S599 K600 K601 Y646 W649 S739 G740 R883 N895 I896 K897 Q944 K945 S982 F983 K984	N161 K168 S169 T170 G243 V246 K252 N257 E258 N261 S287 S289 Y291 K350 W356 N505 T508 Q509 K510 D531 D533 K534 Y538 L581 P583 K591 S595 K596 K597 Y642 W645 S735 G736 R879 N891 I892 K893 Q933 K934 S971 F972 K973
BS03	dna	A	K120 K121 G146 T148 T149 Q529 K591 K595	K121 K122 G147 T149 T150 Q525 K587 K591

Gene Ontology

Molecular Function

• GO:0046872 metal ion binding

External links

• PDB: RCSB:5xus, PDBe:5xus, PDBj:5xus
• PDBsum: 5xus
• PubMed: 28781234
• UniProt: A0A182DWE3