Structure of PDB 5xqa Chain A

Receptor sequence

>5xqaA (length=675) Species: 322104 (Scheffersomyces stipitis CBS 6054)

SVDQKAISTIRLLAVDAVAAANSGHPGAPLGLAPAAHAVFKKMRFNPKDT
KWINRDRFVLSNGHACALLYSMLVLYGYDLTVEDLKKFRQLGSKTPGHPE
NTDVPGAEVTTGPLGQGICNGVGIALAQAQFAATYNKPDFPISDSYTYVF
LGDGCLMEGVSSEASSLAGHLQLGNLIAFWDDNKISIDGSTEVAFTEDVI
ARYKSYGWHIVEVSDADTDITAIAAAIDEAKKVTNKPTLVRLTTTIGFGS
LAQGTHGVHGAPLKADDIKQLKTKWGFNPEESFAVPAEVTASYNEHVAEN
QKIQQQWNELFAAYKQKYPELGAELQRRLDGKLPENWDKALPVYTPADAA
VATRKLSEIVLSKIIPEVPEIIGGSADLTPSNLTKAKGTVDFQPAATGLG
DYSGRYIRYGVREHAMGAIMNGIAAFGANYKNYGGTFLNFVSYAAGAVRL
SALSEFPITWVATHDSIGLGEDGPTHQPIETLAHFRATPNISVWRPADGN
ETSAAYKSAIESTHTPHILALTRQNLPQLEGSSIEKASKGGYTLVQQDKA
DIIIVATGSEVSLAVDALKVLEGQGIKAGVVSLPDQLTFDKQSEEYKLSV
LPDGVPILSVEVMSTFGWSKYSHQQFGLNRFGASGKAPEIFKLFEFTPEG
VAERAAKTVAFYKGKDVVSPLRSAF

3D structure

• PDB: 5xqa Evidence of Diradicals Involved in the Yeast Transketolase Catalyzed Keto-Transferring Reactions.
• Chain: A
• Resolution: 1.14 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H27 I248 H261 E415 H478
• Catalytic site (residue number reindexed from 1): H25 I246 H259 E413 H476
• Enzyme Commision number: 2.2.1.1: transketolase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TPP	A	H66 G114 L116 D155 G156 N185 I187 S188 I189 I248 H261	H64 G112 L114 D153 G154 N183 I185 S186 I187 I246 H259
BS02	CA	A	D155 N185 I187	D153 N183 I185
BS03	HSX	A	H27 I189 H261	H25 I187 H259

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004802 transketolase activity
• GO:0016740 transferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006098 pentose-phosphate shunt

Cellular Component

• GO:0005634 nucleus
• GO:0005829 cytosol

External links

• PDB: RCSB:5xqa, PDBe:5xqa, PDBj:5xqa
• PDBsum: 5xqa
• PubMed: 30155962
• UniProt: P34736|TKT_PICST Transketolase (Gene Name=TKT)