Structure of PDB 5xou Chain A

Receptor sequence

>5xouA (length=564) Species: 262724 (Thermus thermophilus HB27) [Search protein sequence]

LGKTEVFLNRFALRPLNPEELRPWRLEVVLDPPPGREEVYPLLAQVARRA
GGVTVRMGDGLASWSPPEVLVLEGTLARMGQTYAYRLYPKGRRPLDPKDP
GERSVLSALARRLLQERLRRLEGVWVEGLAVYRREVLGGAVLDLWVSDSG
AFLLEVDPALPWEERRRRTREIASWIGRRLGLGTPEAVRAQAYRLSIPKL
MGRRAVSKPADALRVGFYRAQETALALLRLDGAQGWPEFLRRALLRAFGA
SGASLRLHTLHAHPSQGLAFREALRKAKEEGVQAVLVLTPPMAWEDRNRL
KALLLREGLPSQILNVPLREEERHRWENALLGLLAKAGLQVVALSGAYPA
ELAVGFDAGGRESFRFGGAACAVGGDGGHLLWTLPEAQAGERIPQEVVWD
LLEETLWAFRRKAGRLPSRVLLLRNGRVPQDEFALALEALAREGIAYDLV
SVRKSGGGRVYPVQGRLADGLYVPLEDKTFLLLTVHRDFRGTPRPLKLVH
EAGDTPLEALAHQIFHLTRLYPASGFAFPRLPAPLHLADRLVKEVGRLGI
RHLKEVDREKLFFV

3D structure

PDB

5xou Structure/cleavage-based insights into helical perturbations at bulge sites within T. thermophilus Argonaute silencing complexes

Chain

Resolution

2.63 Å

3D
structure

[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]

Enzymatic activity

Enzyme Commision number

3.1.24.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	A	Y43 A170 R286 M413 W415 K422 Q433 I434 L435 N436 R446 N449 R486 E512 R513 P550 Q551 R580 R611 T613 P614 R615 A644 P650 R651 H657 R661 V685	Y40 A159 R165 M292 W294 K301 Q312 I313 L314 N315 R325 N328 R365 E391 R392 P429 Q430 R459 R490 T492 P493 R494 A523 P529 R530 H536 R540 V564
BS02	dna	A	P44 A47 R114 S328 K329 H445 G481 G547 R574 K575 S576 D590 V606 H607 R608 F610 R640 F647 F649 K664 R668	P41 A44 R111 S207 K208 H324 G360 G426 R453 K454 S455 D469 V485 H486 R487 F489 R519 F526 F528 K543 R547
BS03	MG	A	Q433 V685	Q312 V564

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0004520	DNA endonuclease activity
GO:0030145	manganese ion binding
GO:0046872	metal ion binding

	Biological Process
GO:0006260	DNA replication
GO:0044355	clearance of foreign intracellular DNA

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:5xou, PDBe:5xou, PDBj:5xou
PDBsum	5xou
PubMed	28911094
UniProt	Q746M7\|AGO_THET2 Protein argonaute (Gene Name=ago)

[Back to BioLiP]