Structure of PDB 5xbl Chain A

Receptor sequence

>5xblA (length=1320) Species: 301447 (Streptococcus pyogenes serotype M1)

KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPEKKNGLFGNLIALSLGLTPNFK
SNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLS
DILRVITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSK
NGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFD
NGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLA
RGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEK
VLPKHSLLYEYFTVYNELTKVKYLSGEQKKAIVDLLFKTNRKVTVKQLKE
DYFKKIECFDFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLF
EDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKSGK
TILDFLKSDGFANRNFMEDIQKAGDSLHEHIANLAGSPAIKKGILQTVKV
VDELVKVMGRHKPENIVIEMARENQTGQKNSRERMKRIEEGIKELGSQIL
KEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSF
LKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRK
FDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDEN
DKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTA
LIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFF
KTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVK
KTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLII
KLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLK
GSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNK
HRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDAT
LIHQSITGLYETRIDLSQLG

3D structure

• PDB: 5xbl Structural basis of CRISPR-SpyCas9 inhibition by an anti-CRISPR protein
• Chain: A
• Resolution: 3.052 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	K3 K30 K33 I43 K44 K45 N46 G56 E57 A59 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 K76 N77 L101 S104 F105 R115 H116 I122 V126 H129 I135 H160 K163 F164 R165 G166 H167 Y325 H328 H329 R340 F351 F352 Y359 A360 I363 D364 K401 Q402 R403 T404 F405 N407 G408 R447 I448 Y450 G453 P454 L455 A456 S460 F462 W464 I473 M495 N497 K735 L738 K742 R753 Y981 P1090 Q1091 V1100 T1102 G1103 G1104 F1105 I1110 P1112 K1113 R1122 K1123 K1124 Y1131 F1134 M1169 S1172 E1225 L1226 A1227 Q1272 H1349 Q1350 S1351 Y1356	K1 K28 K31 I41 K42 K43 N44 G54 E55 A57 T60 R61 K63 R64 R67 R68 R69 Y70 R72 R73 K74 N75 L99 S102 F103 R113 H114 I120 V124 H127 I133 H158 K161 F162 R163 G164 H165 Y319 H322 H323 R334 F345 F346 Y353 A354 I357 D358 K395 Q396 R397 T398 F399 N401 G402 R441 I442 Y444 G447 P448 L449 A450 S454 F456 W458 I467 M489 N491 K692 L695 K699 R710 Y935 P1044 Q1045 V1054 T1056 G1057 G1058 F1059 I1064 P1066 K1067 R1076 K1077 K1078 Y1085 F1088 M1123 S1126 E1179 L1180 A1181 Q1226 H1303 Q1304 S1305 Y1310

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004520 DNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0008408 3'-5' exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:5xbl, PDBe:5xbl, PDBj:5xbl
• PDBsum: 5xbl
• PubMed: 28448066
• UniProt: Q99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)