Structure of PDB 5x7p Chain A

Receptor sequence

>5x7pA (length=1247) Species: 1117987 (Paenibacillus sp. 598K)

MAGLGNVTGAVASGDSLTLTLDNGTSASDILELDVLSEELLRVDYRPSGA
APSPSTPMIDPDASWDAVGATIDTSGDPIVVTTPRMRIEIARTPARMTIK
KADGTTLLWEPASGGVFEDGVRFQRGSTDNIYGIRSFNAQEDVGGLLRNS
SDHPAHAGQQGDAGGPFMWSTAGYGVLVDSDGGYPYTDTTGKLEFYYGGT
PTEGRRYTKTNVEYYIMVGEPKEIMASYAQVTGTAPMLPKWSLGFMNFEW
GIDQDELEAHVDGYRARNIPIDAFALDYDWMDYGEDNYGEFRWNTDNFPD
AATTQLKEDMEAEGIRLIGIRKPRIITRDFANQRTQQYYDADSNGYFYPG
HNEYTDYFIPVTVRSFDPYQQASRDWWWQHSIDAFDKGIVGWWNDETDKV
DSGSAQYWFGNFSTGFTSQAMYDGQRDYTNDGVRVWQTARSYYPGAQRYA
TTLWSGDIGTQFYKGELFNWAPGMQEQPRIMLSSANLGQPKWGMDTGGFN
SLGGASGPNPSPELYTRWMQFGAFTPVFRVHGNYNQQRQPWLYGATAEEA
SKAVMHTRYSLLPYMYAYEREASETGLGLIKPLLFDYPNDPQAADYTEAW
MFGDWLLVSPVLGEAQHSKQIYLPAGTWIDYHRGQTYSGGQTIHYPVNAD
TWTDVPLFVKQGAIIPNQQVLDYVDQQSVTTVNVDIFPSASETSFTYYED
DGSSYDYESGSSFEQRLAAQDLSSSVRVEVGAGSGSYTPDVQHYVLKIHG
RAGSAVTAGGSALTGYGDLQALQAASGSGWASGRDIYGDVTYVKLPAASG
SATVVEVSGSAPSAATHAIYEVEDASRSGATPTTRAGINTNHSGYSGSGF
VDKLDVPGAAVTVYANAPVSGDYPVELRYANGSGSAKTLSVYVNAARVQQ
LSLADTGAWSQWGTQTTTLPLTAGQNIITYKYDSDAGDTGGVNLDYIRVP
FAPTQAEYAAESAKLWGGAGTSQDHWFYKGAAFVDNLTGVGAEASFDVYA
PSAGTYNLSLRYANGTGSTKTLSAIVNGGAASTVTLTSPGMNWNLWNEHT
MTATLTAGRNTISFRRNSGNSGNVNLDRLAVSASAITTLASERNLLDNGD
FERDTTYNSNWTQWQPSGQPSAFGIDSGNALHPPEGPARRNQRAYFHSDN
AYQQSIHQVVDVPVNNATYRLEAKVRMKNTTPTTARAEVQGHGGSPIYAN
ISNDGVWKTIVIDNINVTSGSVDVGFYVDSPGYTTLHIDEVTLTRAP

3D structure

• PDB: 5x7p Carbohydrate-binding architecture of the multi-modular alpha-1,6-glucosyltransferase from Paenibacillus sp. 598K, which produces alpha-1,6-glucosyl-alpha-glucosaccharides from starch
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: '2.4.1.-
  3.2.1.20'

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	D491 N534	D457 N500
BS02	AC1	A	D429 E430 R474 D491 F533 H565	D395 E396 R440 D457 F499 H531
BS03	GLC	A	Y218 G232 G233	Y184 G198 G199
BS04	AC1	A	H876 W943 N977	H842 W909 N943
BS05	AC1	A	W1148 S1189 H1191 R1220 E1222 Y1261	W1114 S1155 H1157 R1186 E1188 Y1227
BS06	AC1	A	D1160 E1169 R1177 Y1179 N1213 T1269	D1126 E1135 R1143 Y1145 N1179 T1235
BS07	GLC	A	Q174 G437 S438	Q140 G403 S404
BS08	AC1	A	W284 D311 Y312 K356	W250 D277 Y278 K322
BS09	CA	A	E855 E857 S880 G883 D979	E821 E823 S846 G849 D945
BS10	CA	A	E995 K1013 A1016 D1111	E961 K979 A982 D1077
BS11	CA	A	D1134 E1136 R1173 Q1176 D1273	D1100 E1102 R1139 Q1142 D1239
BS12	MG	A	E283 G285	E249 G251

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0016787 hydrolase activity
• GO:0030246 carbohydrate binding
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:5x7p, PDBe:5x7p, PDBj:5x7p
• PDBsum: 5x7p
• PubMed: 28698247
• UniProt: A0A193PKW5