Structure of PDB 5x3j Chain A

Receptor sequence

>5x3jA (length=718) Species: 479435 (Kribbella flavida DSM 17836)

MIKHRPHGIEHPYAVSPDQRVPVLPLAGEPVLLGVVAPEADRVVCEWGTL
ELPLSATSAAAADAAALAGGEGHLSEAQADGAWSVQTPPLAEPVKYRFHA
HRGGAAESTEWFEVSPAVWTADGVGEVRGGGERVRGVEWLVSSQGVHRGR
FRLQLQDGDRLVGFGERYDALDQRGRELDAVVFEQYKAQGVHGRTYLPMP
FAHVVGADGNGWGFHVRTSRRTWYSSAGNELTVEVALGDEPVVDLAIYEG
DPATVLTGFLDEVGRAEELPGWVFRLWASGNEWNTQQLVTARMDTHRDLA
IPVGAVVIEAWSDEQGITIWRDAVYAVTEDGSAHRAEDFSYRPDGAWPDP
KAMIDELHARGIKVILWQIPLQKTEFSTGQVAADAAAMVRDGHAVLEADG
TAYRNRGWWFPQALMPDLSVQRTRDWWTEKRRYLVEHFDVDGFKTAGGEH
AWGHDLVYADGRKGDEGNNLYPVHYARAFGDLLRSAGKAPVTFSRAGFTG
SQAHGIFWAGDEDSTWQAFRSSVTAGLTAASCGIVYWGWDLAGFSGPVPD
AELYLRAAAASAFMPIMQYHSEFNHHQLPLRDRTPWHVAETTGDDRVVPL
FRRFATLRESLVPYLTEQAARTIATDRPLMRPLFFDHENDPEIWNHPYQY
LLGDELLINPVLEPGATTWTTYLPAGEWIDVWTGDRVPSGLVTRDVPLEV
VPVYCRASRWSELQPVFS

3D structure

• PDB: 5x3j Glycoside hydrolase mutant in complex with substrate
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.204: 1,3-alpha-isomaltosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	H73 E189 Y191 W413 W414 R500 D516 E577	H73 E184 Y186 W408 W409 R495 D511 E572
BS02	GLC	A	E314 W414 R500 W513 D516 F549 H575	E309 W409 R495 W508 D511 F544 H570
BS03	GLC	A	N286 E319 W372 F578	N281 E314 W367 F573

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0030246 carbohydrate binding

Biological Process

• GO:0000272 polysaccharide catabolic process
• GO:0005975 carbohydrate metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5x3j, PDBe:5x3j, PDBj:5x3j
• PDBsum: 5x3j
• UniProt: D2PPM7|AIMA_KRIFD 1,3-alpha-isomaltosidase (Gene Name=Kfla_1895)