Structure of PDB 5x2h Chain A

Receptor sequence

>5x2hA (length=746) Species: 192222 (Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819) [Search protein sequence]

MARILAFDIGISSIGWAFSENDELKDCGVRIFTESLALPRRLARSARKRL
ARRKARLNHLKHLIANEFKLNYEDYQSFDESLAKAYKGSLISPYELRFRA
LNELLSKQDFARVILHIAKRRGYDDIKNKEKGAILKAIKQNEEKLANYQS
VGEYLYKEYFQKFKENSKEFTNVRNKKESYERCIAQSFLKDELKLIFKKQ
REFGFSFSKKFEEEVLSVAFYKRALKDFSHLVGNCSFFTDEKRAPKNSPL
AFMFVALTRIINLLNNLKNTEGILYTKDDLNALLNEVLKNGTLTYKQTKK
LLGLSDDYEFKGEKGTYFIEFKKYKEFISQDDLNEIAKDITLIKDEIKLK
KALAKYDLNQNQIDSLSKLEFKDHLNISFKALKLVTPLMLEGKKYDEACN
ELNLKVAINEDKKDFLPAFNETYYKDEVTNPVVLRAIKEYRKVLNALLKK
YGKVHKINIELGGYIARLVLNYTKDYLDFLPLSDVHVEAKSGMLTSALRH
TWGFSAKDRNNHLHHAIDAVIIAYRQKVLDKIDEIFVSKPERKKPSGALH
EETFRKEEEFYQSYGGKEGVLKALELGKIRKVNGKIVKNGDMFRVDIFKH
KKTNKFYAVPIYTMDFALKVLPNKAVARSKKGEIKDWILMDENYEFCFSL
YKDSLILIQTKDMQEPEFVYYNAFTSSTVSLIVSKHDNKFETLSKNQKIL
FKNANEKEVIAKSIGIQNLKVFEKYIVSALGEVTKAEFRQREDFKK

3D structure

PDB

5x2h Crystal Structure of the Minimal Cas9 from Campylobacter jejuni Reveals the Molecular Diversity in the CRISPR-Cas9 Systems

Chain

Resolution

2.3 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	rna	A	S43 L44 A45 R48 R49 A51 R52 R55 K56 R57 L58 R60 R61 K62 A63 N66 H67 K69 H70 N74 S85 L90 Y94 G96 S97 P101 Y102 H124 K127 R128 R129 G130 Y131 Q172 E180 F181 V184 R185 N186 K187 K233 R234 A235 L236 K237 S247 K257 F265 V266 L394 N395 Y414 T448 N449 P450 R454 K457 R460 K461 R645 E779 R780 K781 K782 P783 S784 G785 A786 L787 H788 F792 N821 K823 F854 A855 I964 S966 K973 A974 E975 E980 D981 F982	S35 L36 A37 R40 R41 A43 R44 R47 K48 R49 L50 R52 R53 K54 A55 N58 H59 K61 H62 N66 S77 L82 Y86 G88 S89 P93 Y94 H116 K119 R120 R121 G122 Y123 Q161 E169 F170 V173 R174 N175 K176 K222 R223 A224 L225 K226 S236 K246 F254 V255 L375 N376 Y395 T429 N430 P431 R435 K438 R441 K442 R467 E541 R542 K543 K544 P545 S546 G547 A548 L549 H550 F554 N583 K585 F616 A617 I726 S728 K735 A736 E737 E742 D743 F744
BS02	dna	A	Y131 K135 G143 I145 L146 Y191 L236 L242 G244 R254 N273 N277 N280 E320 K363 D392 H393 K413 D415 Y642 E789 E790 T791 R793 R866 T916 K940 N941 S951 G953 N956	Y123 K127 G132 I134 L135 Y180 L225 L231 G233 R243 N262 N266 N269 E309 K344 D373 H374 K394 D396 Y464 E551 E552 T553 R555 R628 T678 K702 N703 S713 G715 N718
BS03	dna	A	R780 S801 Y802 N827 M830 Y850 K890 T913 S915	R542 S563 Y564 N589 M592 Y612 K652 T675 S677

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0004519	endonuclease activity

View graph for
Molecular Function

External links

PDB	RCSB:5x2h, PDBe:5x2h, PDBj:5x2h
PDBsum	5x2h
PubMed	28306506
UniProt	Q0P897\|CAS9_CAMJE CRISPR-associated endonuclease Cas9 (Gene Name=cas9)

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