Structure of PDB 5x2g Chain A

Receptor sequence

>5x2gA (length=749) Species: 192222 (Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819) [Search protein sequence]

MARILAFDIGISSIGWAFSENDELKDCGVRIFTKGESLALPRRLARSARK
RLARRKARLNHLKHLIANEFKLNYEDYQSFDESLAKAYKGSLISPYELRF
RALNELLSKQDFARVILHIAKRRGYDDIKNKEKGAILKAIKQNEEKLANY
QSVGEYLYKEYFQKFKENSKEFTNVRNKKESYERCIAQSFLKDELKLIFK
KQREFGFSFSKKFEEEVLSVAFYKRALKDFSHLVGNCSFFTDEKRAPKNS
PLAFMFVALTRIINLLNNLKNTEGILYTKDDLNALLNEVLKNGTLTYKQT
KKLLGLSDDYEFKGEKGTYFIEFKKYKEFISQDDLNEIAKDITLIKDEIK
LKKALAKYDLNQNQIDSLSKLEFKDHLNISFKALKLVTPLMLEGKKYDEA
CNELNLKVAINEDKKDFLPAFNETYYKDEVTNPVVLRAIKEYRKVLNALL
KKYGKVHKINIELGGGYIARLVLNYTKDYLDFLPLSDVHVEAKSGMLTSA
LRHTWGFSAKDRNNHLHHAIDAVIIAYRQKVLDKIDEIFVSKPERKKPSG
ALHEETFRKEEEFYQSYGGKEGVLKALELGKIRKVNGKIVKNGDMFRVDI
FKHKKTNKFYAVPIYTMDFALKVLPNKAVARSKKGEIKDWILMDENYEFC
FSLYKDSLILIQTKDMQEPEFVYYNAFTSSTVSLIVSKHDNKFETLSKNQ
KILFKNANEKEVIAKSIGIQNLKVFEKYIVSALGEVTKAEFRQREDFKK

3D structure

PDB

5x2g Crystal Structure of the Minimal Cas9 from Campylobacter jejuni Reveals the Molecular Diversity in the CRISPR-Cas9 Systems

Chain

Resolution

2.4 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	rna	A	A45 R48 R49 A51 R52 R55 K56 R57 L58 R60 R61 K62 A63 N66 H67 K69 H70 N74 S85 E88 L90 Y94 G96 S97 L98 P101 Y102 H124 K127 R128 R129 G130 Y131 Q172 E180 F181 T182 V184 R185 N186 K187 K233 R234 A235 L236 K237 S247 K257 F265 V266 T269 R270 L394 N395 Y414 T448 N449 P450 R454 K457 R460 K461 R645 E779 K781 K782 P783 G785 A786 L787 H788 F792 K794 N821 K823 F854 A855 I964 S966 E975 R977 E980 D981 F982	A39 R42 R43 A45 R46 R49 K50 R51 L52 R54 R55 K56 A57 N60 H61 K63 H64 N68 S79 E82 L84 Y88 G90 S91 L92 P95 Y96 H118 K121 R122 R123 G124 Y125 Q163 E171 F172 T173 V175 R176 N177 K178 K224 R225 A226 L227 K228 S238 K248 F256 V257 T260 R261 L377 N378 Y397 T431 N432 P433 R437 K440 R443 K444 R470 E544 K546 K547 P548 G550 A551 L552 H553 F557 K559 N586 K588 F619 A620 I729 S731 E740 R742 E745 D746 F747
BS02	dna	A	Y131 K135 G143 I145 L146 Y191 L236 F239 L242 G244 R254 N273 N277 N280 E320 K363 D392 H393 K413 D415 E446 Y642 E790 T791 R793 R866 T916 K940 S951	Y125 K129 G134 I136 L137 Y182 L227 F230 L233 G235 R245 N264 N268 N271 E311 K346 D375 H376 K396 D398 E429 Y467 E555 T556 R558 R631 T681 K705 S716
BS03	dna	A	R780 S801 Y802 N827 G828 M830 Y850 K890 T913 S915	R545 S566 Y567 N592 G593 M595 Y615 K655 T678 S680

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0004519	endonuclease activity

View graph for
Molecular Function

External links

PDB	RCSB:5x2g, PDBe:5x2g, PDBj:5x2g
PDBsum	5x2g
PubMed	28306506
UniProt	Q0P897\|CAS9_CAMJE CRISPR-associated endonuclease Cas9 (Gene Name=cas9)

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