Structure of PDB 5wwr Chain A

Receptor sequence

>5wwrA (length=461) Species: 9606 (Homo sapiens) [Search protein sequence]

SIFPKISLRPEVENYLKEGFMNKEIVTALGKQEAERKFETLLKHLSHPPS
FTTVRVNTHLASVQHVKNLLLDELQKQFNGLSVPILQHPDLQDVLLIPVI
GPRKNIKKQQCEAIVGAQCGNAVLRGAHVYAPGIVSASQFMKAGDVISVY
SDIKGKCKKGAKEFDGTKVFLGNGISELSRKEIFSGLPELKGMGIRMTEP
VYLSPSFDSVLPRYLFLQNLPSALVSHVLNPQPGEKILDLCAAPGGKTTH
IAALMHDQGEVIALDKIFNKVEKIKQNALLLGLNSIRAFCFDGTKAVKLD
MVGEPPFLPESFDRILLDAPCSGMGQRPNMACTWSVKEVASYQPLQRKLF
TAAVQLLKPEGVLVYSTCTITLAENEEQVAWALTKFPCLQLQPQEPQIGG
EGMRGAGLSCEQLKQLQRFDPSAVPLPDTDMDSLREARREDMLRLANKDS
IGFFIAKFVKC

3D structure

PDB

5wwr Structural basis for substrate binding and catalytic mechanism of a human RNA:m5C methyltransferase NSun6

Chain

Resolution

3.096 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.1.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	T53 T54 Q119 C120 A123 R126 G127 A128 Y131 P133 G134 F141 K159 K160 R181 K182 F185 G187 L188 K192 G193 F208 D209 L218 Q219 N220 S223 P245 K248 I268 D323 Q331 E343 S346 Y347 P349 R352 K353 S371 C373	T52 T53 Q118 C119 A122 R125 G126 A127 Y130 P132 G133 F140 K158 K159 R180 K181 F184 G186 L187 K191 G192 F207 D208 L217 Q218 N219 S222 P244 K247 I267 D318 Q326 E338 S341 Y342 P344 R347 K348 S366 C368
BS02	SFG	A	C242 A243 A244 K248 D266 K271 G294 D323	C241 A242 A243 K247 D265 K270 G293 D318

Gene Ontology

	Molecular Function
GO:0000049	tRNA binding
GO:0003723	RNA binding
GO:0008168	methyltransferase activity
GO:0008173	RNA methyltransferase activity
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
GO:0016428	tRNA (cytidine-5-)-methyltransferase activity

	Biological Process
GO:0001510	RNA methylation
GO:0002946	tRNA C5-cytosine methylation
GO:0006400	tRNA modification
GO:0030488	tRNA methylation
GO:0032259	methylation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:5wwr, PDBe:5wwr, PDBj:5wwr
PDBsum	5wwr
PubMed	28531330
UniProt	Q8TEA1\|NSUN6_HUMAN tRNA (cytosine(72)-C(5))-methyltransferase NSUN6 (Gene Name=NSUN6)

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