Structure of PDB 5wtk Chain A

Receptor sequence

>5wtkA (length=1215) Species: 1122172 (Leptotrichia shahii DSM 19757)

GNLFGHKRWYEVRDKKDFKIKRKVKVKRNYDGNKYILNINENNNKEKIDN
NKFIRKYINYKKNDNILKEFTRKFHAGNILFKLKGKEGIIRIENNDDFLE
TLRIIENDELETKKSIYEIFKNINMSLYKIIEKIIENETEKVFENRYYEE
HLREKLLKDDKIDVILTNFMEIREKIKSNLEILGFVKFYLNVGGDKKKSK
NKKMLVEKILNINVDLTVEDIADFVIKELEFWNITKRIEKVKKVNNEFLE
KRRNRTYIKSYVLLDKHEKFKIERENKKDKIVKFFVENIKNNSIKEKIEK
ILAEFKIDELIKKLEKELKKGNCDTEIFGIFKKHYKVNFDSKKFSKKSDE
EKELYKIIYRYLKGRIEKILVNEQKVRLKKMEKIEIEKILNESILSEKIL
KRVKQYTLEHIMYLGKLRHNDIDMTTVNTDDFSRLHAKEELDLELITFFA
STNMELNKIFSRENINNDENIDFFGGKNYVLDKKILNSKIKIIRDLDFID
NKNNITNNFIRKFTKIGTNERNRILHAISKERDLQGTQDDYNKVINIIQN
LKISDEEVSKALNLDVVFKDKKNIITKINDIKISEENNNDIKYLPSFSKV
LPEILNLYRNNPKNEPFDTIETEKIVLNALIYVNKELYKKLILEDDLEEN
ESKNIFLQELKKTLGNIDEIDENIIENYYKNAQISASKGNNKAIKKYQKK
VIECYIGYLRKNYEELFDFSDFKMNIQEIKKQIKDINDNKTYERITVKTS
DKTIVINDDFEYIISIFALLNSNAVINKIRNRFFATSVWLNTSEYQNIID
ILDEIMQLNTLRNECITENWNLNLEEFIQKMKEIEKDIKSKILCRIIFNS
DFLKKYKKEIDNLIEDMESENENKFQEIYYPKERKNELYIYKKNLFLNIG
NPNFDKIYGLISNDIKMADAKFLFNIDGKNIRKNKISEIDAILKNLNDKL
NGYSKEYKEKYIKKLKENDDFFAKNIQNKNYKSFEKDYNRVSEYKKIRDL
VEFNYLNKIESYLIDINWKLAIQMARFERDMHYIVNGLRELGIIKLSGYN
TGISRAYPKRNGSDGFYTTTAYYKFFDEESYKKFEKICYGFGIDLSENSE
INKPENESIRNYISHFYIVRNPFADYSIAEQIDRVSNLLSYSTRYNNSTY
ASVFEVFKKDVNLDYDELKKKFKLIGNNDILERLMKPKKVSVLELESYNS
DYIKNLIIELLTKIE

3D structure

• PDB: 5wtk Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities
• Chain: A
• Resolution: 2.651 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	N3 L4 Y58 K62 R73 H76 K85 I91 R219 D268 E312 K315 T329 Y330 S333 Y334 K339 H340 K342 T398 F401 K405 Y408 S414 Y428 K429 Y432 R433 K436 K471 R475 Q478 E482 T502 F505 S506 H509 S672 S674 K675 Q759 A762 S763 N767 I770 K771 N849 A850 N853 N857 R858 W865 M882 R1194 T1311 R1312 N1315 S1316 E1323 K1337 V1358 V1360	N2 L3 Y57 K61 R72 H75 K84 I90 R146 D195 E239 K242 T256 Y257 S260 Y261 K266 H267 K269 T325 F328 K332 Y335 S341 Y355 K356 Y359 R360 K363 K398 R402 Q405 E409 T429 F432 S433 H436 S596 S598 K599 Q683 A686 S687 N691 I694 K695 N773 A774 N777 N781 R782 W789 M806 R1026 T1143 R1144 N1147 S1148 E1155 K1169 V1190 V1192

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:5wtk, PDBe:5wtk, PDBj:5wtk
• PDBsum: 5wtk
• PubMed: 28086085
• UniProt: P0DOC6|CS13A_LEPSD CRISPR-associated endoribonuclease Cas13a (Gene Name=cas13a)