Structure of PDB 5wqe Chain A

Receptor sequence

>5wqeA (length=1011) Species: 1356854 (Alicyclobacillus acidoterrestris ATCC 49025)

AVKSIKVKLRLDDMPEIRAGLWKLHKEVNAGVRYYTEWLSLLRQENLYRR
SPQECDKTAEECKAELLERLRARQVENGHRGPAGSDDELLQLARQLYELL
VPQAIGAKGDAQQIARKFLSPLADKDAVRTADVLRALADFGLKPLMRVYT
DSEMSSVEWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGQEYAKL
VEQKNRFEQKNFVGQEHLVHLVNQLQQDMKEATGRALRGSDKVFEKWGKL
APDAPFDLYDAEIKNVQRRNTRRFGSHDLFAKLAEPEYQALWREDASFLT
RYAVYNSILRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNLHQYTFLFN
EFGERRHAIRFHKLLKVENGVAREVDDVTVPISMSEQLDNLLPRDPNEPI
ALYFRDYGAEQHFTGEFGGAKIQCRRDQLAHMHGARDVYLNVSVRVQSQS
EARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDGKLGSEGLLS
GLRVMSVDLGLRTSASISVFRVARKDELKPNSKGRVPFFFPIKGNDNLVA
VHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLRLLVRCGSED
VGRRERSWAKLIEQPVDAANHMTPDWREAFENELQKLKSLHGICSDKEWM
DAVYESVRRVWRHMGKQVRDWRKDVRSGERPKIRGYAKDVVGGNSIEQIE
YLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDHAKEDRLKKL
ADRIIMEALGYVYALDERGKGKWVAKYPPCQLILLEELSEYQFNNDRPPS
ENNQLMQWSHRGVFQELINQAQVHDLLVGTMYAAFSSRFDARTGAPGIRC
RRVADDLIPTGEGEIFVSPFSAEEGDFHQIHADLNAAQNLQQRLWSDFDI
SQIRLRCDWGEVDGELVLIPRLTGKRTADSYSNKVFYTNTGVTYYERERE
KLSEEEAELLVEADEAREKSVVLMRDPSGIINRGNWTRQKEFWSMVNQRI
EGYLVKQIRSR

3D structure

• PDB: 5wqe C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism
• Chain: A
• Resolution: 3.126 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	V3 K4 S5 K7 K9 W234 S442 M443 S444 Q446 R484 R485 Y501 N503 R597 F600 H614 Q618 L619 I634 W723 G727 V730 R731 R742 P743 K744 I745 R746 G747 V752 V753 Q767 L771 W774 S775 S791 F793 A794 T796 L797 H800 H803 D807 K810 K811 D814 R815 E819 Y825 Y839 N881 Q882 H972 Q973	V2 K3 S4 K6 K8 W189 S383 M384 S385 Q387 R425 R426 Y439 N441 R535 F538 H552 Q556 L557 I572 W661 G665 V668 R669 R680 P681 K682 I683 R684 G685 V690 V691 Q705 L709 W712 S713 S729 F731 A732 T734 L735 H738 H741 D745 K748 K749 D752 R753 E757 Y763 Y777 N819 Q820 H878 Q879

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:5wqe, PDBe:5wqe, PDBj:5wqe
• PDBsum: 5wqe
• PubMed: 27989439
• UniProt: T0D7A2|CS12B_ALIAG CRISPR-associated endonuclease Cas12b (Gene Name=cas12b)