Structure of PDB 5wp5 Chain A

Receptor sequence

>5wp5A (length=463) Species: 3702 (Arabidopsis thaliana)

EERDIQKSYWMEHRPEVLSLIPPYEGKSVLELGAGIGRFTGELAQKAGEV
IALDFIESAIQKNESVNGHYKNIKFMCADVTSPDLKIKDGSIDLIFSNWL
LMYLSDKEVELMAERMIGWVKPGGYIFFRESCFHQSGDSKRKSNPTHYRE
PRFYTKVFQECQTRDASGNSFELSMVGCKCIGAYVKNKKNQNQICWIWQK
VSVENDKDFQRFLDNVQYKSSGILRYERVFGEGYVSTGGFETTKEFVAKM
DLKPGQKVLDVGCGIGGGDFYMAENFDVHVVGIDLSVNMISFALERAIGL
KCSVEFEVADCTTKTYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLKPG
GKVLITDYCRSAETPSPEFAEYIKQRGYDLHDVQAYGQMLKDAGFDDVIA
EDRTDQFVQVLRRELEKVEKEKEEFISDFSEEDYNDIVGGWSAKLERTAS
GEQKWGLFIADKK

3D structure

• PDB: 5wp5 Conformational changes in the di-domain structure of Arabidopsis phosphoethanolamine methyltransferase leads to active-site formation.
• Chain: A
• Resolution: 1.5 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.103: phosphoethanolamine N-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SAH	A	V263 S264 G290 G292 D312 L313 M317 D338 C339 R355 T357 H360	V235 S236 G262 G264 D284 L285 M289 D310 C311 R327 T329 H332	MOAD: Kd=4.6uM PDBbind-CN: -logKd/Ki=5.34,Kd=4.6uM
BS02	SAH	A	W16 G61 R66 F67 D82 F83 D107 V108 N126 W127	W10 G33 R38 F39 D54 F55 D79 V80 N98 W99	MOAD: Kd=4.6uM PDBbind-CN: -logKd/Ki=5.34,Kd=4.6uM

Gene Ontology

Molecular Function

• GO:0000234 phosphoethanolamine N-methyltransferase activity
• GO:0003729 mRNA binding
• GO:0008168 methyltransferase activity

Biological Process

• GO:0006656 phosphatidylcholine biosynthetic process
• GO:0008654 phospholipid biosynthetic process
• GO:0009058 biosynthetic process
• GO:0032259 methylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5wp5, PDBe:5wp5, PDBj:5wp5
• PDBsum: 5wp5
• PubMed: 29084845
• UniProt: Q944H0|PEAM2_ARATH Phosphoethanolamine N-methyltransferase 2 (Gene Name=NMT2)