Structure of PDB 5wm8 Chain A

Receptor sequence

>5wm8A (length=432) Species: 559292 (Saccharomyces cerevisiae S288C)

IVACDDPDFLTSYFAHSRLHHLSAWKANLKDKFLNENIHKYTKITDKDTY
IIFHIDFDCFFATVAYLCRSSSFSACDFKRDPIVVCHGTKNSDIASCNYV
ARSYGIKNGMWVSQAEKMLPNGIKLISLPYTFEQFQLKSEAFYSTLKRLN
IFNLILPISIDEAVCVRIIPDNIHNTNTLNARLCEEIRQEIFQGTNGCTV
SIGCSDSLVLARLALKMAKPNGYNITFKSNLSEEFWSSFKLDDLPGVGHS
TLSRLESTFDSPHSLNDLRKRYTLDALKASVGSKLGMKIHLALQGQDDEE
SLKILYDPKEVLQRKSLSIDINWGIRFKNITQVDLFIERGCQYLLEKLNE
INKTTSQITLKLMRRCKDAPIEPPKYMGMGRCDSFSRSSRLGIPTNEFGI
IATEMKSLYRTLGCPPMELRGLALQFNKLVDV

3D structure

• PDB: 5wm8 Mechanism of error-free replication across benzo[a]pyrene stereoisomers by Rev1 DNA polymerase.
• Chain: A
• Resolution: 1.92 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	I464 G552 G554 H555 S556 T557 S692 R693 S694 R696	I158 G246 G248 H249 S250 T251 S386 R387 S388 R390
BS02	dna	A	I307 Y319 F320 H322 S323 R324 L325 H326 S329 H393 G394 T395 K396 N397 S398 D399 K590 R620 K621 S624 I625 D626 I627 N628 W629 Y682 G684 M685 G686	I1 Y13 F14 H16 S17 R18 L19 H20 S23 H87 G88 T89 K90 N91 S92 D93 K284 R314 K315 S318 I319 D320 I321 N322 W323 Y376 G378 M379 G380
BS03	MG	A	D548 L550 V553	D242 L244 V247
BS04	MG	A	D467 E468	D161 E162
BS05	MG	A	D362 F363 D467	D56 F57 D161
BS06	DCP	A	R324 L328 D362 F363 D364 C365 F366 F367 A401 S402 Y405 R408 N414 D467 K525	R18 L22 D56 F57 D58 C59 F60 F61 A95 S96 Y99 R102 N108 D161 K219

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:5wm8, PDBe:5wm8, PDBj:5wm8
• PDBsum: 5wm8
• PubMed: 29042535
• UniProt: P12689|REV1_YEAST DNA repair protein REV1 (Gene Name=REV1)