Structure of PDB 5w7e Chain A

Receptor sequence

>5w7eA (length=521) Species: 10090 (Mus musculus)

SHGQSCLGCVVLVSVIEQLAEVHNSSVQVAMERLCSYLPEKLFLKTACYF
LVQTFGSDIIKLLDEAMKADVVCYALEFCKRQCHLYPLPQEAWESALEKA
RQVLICSLPFLTKICQKIELSIKKAVPFKDVDSDKHSVFPTLRGYHWRGR
DCNDSDKTVYPGRRPDNWDIHQDSNCNGIWGIDPKDGIPYEKKFCEGSQP
RGIILLGDAAGAHFHIPPEWLTASQMSVNSFLNLPSALTDELNWPQLSGV
TGFLDSTSGIEEKSIYHRLRKRNHCNHRDYQSISKNGASSRNLKNFIESL
SRNQASDHPAIVLYAMIGNDVCNSKADTVPEMTTPEQMYANVMQTLTHLN
SHLPNGSHVILYGLPDGTFLWDSLHNRYHPLGQLNKDVTYAQFFSFLRCL
QLNPCNGWMSSNKTLRTLTSERAEQLSNTLKKIATTETFANFDLFYVDFA
FHEIIEDWQKRGGQPWQLIEPVDGFHPNEVASLLQANRVWEKIQLQWPHV
LGKENPFNSQIEEVFGDQGGH

3D structure

• PDB: 5w7e Crystal structure of the mammalian lipopolysaccharide detoxifier.
• Chain: A
• Resolution: 1.83 Å

Enzymatic activity

• Enzyme Commision number: 3.1.1.77: acyloxyacyl hydrolase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	D183 D185 D187 H189 D204 D207	D130 D132 D134 H136 D151 D154
BS02	CA	A	D185 D187 D204 N206 D209 V212	D132 D134 D151 N153 D156 V159
BS03	CA	A	D222 D226 N228 N230 I232 E244	D169 D173 N175 N177 I179 E191
BS04	FAW	A	D261 A262 G340 N372 F422 L423 F504 F528 H529 P530	D208 A209 G287 N319 F369 L370 F451 F475 H476 P477

Gene Ontology

Molecular Function

• GO:0005509 calcium ion binding
• GO:0016787 hydrolase activity
• GO:0016788 hydrolase activity, acting on ester bonds
• GO:0046872 metal ion binding
• GO:0050528 acyloxyacyl hydrolase activity

Biological Process

• GO:0006629 lipid metabolic process
• GO:0006631 fatty acid metabolic process
• GO:0008653 lipopolysaccharide metabolic process
• GO:0009104 lipopolysaccharide catabolic process
• GO:0050728 negative regulation of inflammatory response

Cellular Component

• GO:0005576 extracellular region
• GO:0031410 cytoplasmic vesicle

External links

• PDB: RCSB:5w7e, PDBe:5w7e, PDBj:5w7e
• PDBsum: 5w7e
• PubMed: 29343645
• UniProt: O35298|AOAH_MOUSE Acyloxyacyl hydrolase (Gene Name=Aoah)