Structure of PDB 5w7d Chain A

Receptor sequence

>5w7dA (length=528) Species: 10090 (Mus musculus)

SHGQSCLGCVVLVSVIEQLAEVHNSSVQVAMERLCSYLPEKLFLKTACYF
LVQTFGSDIIKLLDEAMKADVVCYALEFCKRGAVQPQCHLYPLPQEAWES
ALEKARQVLRRSCSLPFLTKICQKIELSIKKAVPFKDVDSDKHSVFPTLR
GYHWRGRDCNDSDKTVYPGRRPDNWDIHQDSNCNGIWGIDPKDGIPYEKK
FCEGSQPRGIILLGDAAGAHFHIPPEWLTASQMSVNSFLNLPSALTDELN
WPQLSGVTGFLDSTSGIEEKSIYHRLRKRNHCNHRDYQSISKNGASSRNL
KNFIESLSRNQASDHPAIVLYAMIGNDVCNSKADTVPEMTTPEQMYANVM
QTLTHLNSHLPNGSHVILYGLPDGTFLWDSLHNRYHPLGQLNKDVTYAQF
FSFLRCLQLNPCNGWMSSNKTLRTLTSERAEQLSNTLKKIATTETFANFD
LFYVDFAFHEIIEDWQKRGGQPWQLIEPVDGFHPNEVASLLQANRVWEKI
QLQWPHVLGKENPFNSQIEEVFGDQGGH

3D structure

• PDB: 5w7d Crystal structure of the mammalian lipopolysaccharide detoxifier.
• Chain: A
• Resolution: 1.75 Å

Enzymatic activity

• Enzyme Commision number: 3.1.1.77: acyloxyacyl hydrolase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	D183 D185 D187 H189 D204 D207	D137 D139 D141 H143 D158 D161
BS02	CA	A	D185 D187 D204 N206 D209 V212	D139 D141 D158 N160 D163 V166
BS03	CA	A	D222 D226 N228 N230 I232 E244	D176 D180 N182 N184 I186 E198

Gene Ontology

Molecular Function

• GO:0005509 calcium ion binding
• GO:0016787 hydrolase activity
• GO:0016788 hydrolase activity, acting on ester bonds
• GO:0046872 metal ion binding
• GO:0050528 acyloxyacyl hydrolase activity

Biological Process

• GO:0006629 lipid metabolic process
• GO:0006631 fatty acid metabolic process
• GO:0008653 lipopolysaccharide metabolic process
• GO:0009104 lipopolysaccharide catabolic process
• GO:0050728 negative regulation of inflammatory response

Cellular Component

• GO:0005576 extracellular region
• GO:0031410 cytoplasmic vesicle

External links

• PDB: RCSB:5w7d, PDBe:5w7d, PDBj:5w7d
• PDBsum: 5w7d
• PubMed: 29343645
• UniProt: O35298|AOAH_MOUSE Acyloxyacyl hydrolase (Gene Name=Aoah)