Structure of PDB 5w66 Chain A

Receptor sequence
>5w66A (length=1461) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
MDISKPVGSEITSVDFGILTAKEIRNLSAKQITNPTVLDNLGHPVSGGLY
DLALGAFLRNLCSTCGLDEKFCPGHQGHIELPVPCYNPLFFNQLYIYLRA
SCLFCHHFRLKSVEVHRYACKLRLLQYGLIDESYKLDEITLDISSTLLNE
LKSKRSEYVDMAIAKALSDGRTTERGSFTATVNDERKKLVHEFHKKLLSR
GKCDNCGMFSPKFRKDGFTKIFETALNEKQITNNRVKGSTYILSTEVKNI
LDTVFRKEQCVLQYVFHSRPNLSRKLVKADSFFMDVLVVPPTRFRLPSKL
GEEVHENSQNQLLSKVLTTSLLIRDLNDDLSKLQKDKVSLEDRRVIFSRL
MNAFVTIQNDVNAFIDSTKAQKVPIPGVKQALEKKEGLFRKHMMGKRVNY
AARSVISPDPNIETNEIGVPPVFAVKLTYPEPVTAYNIAELRQAVINGPD
KWPGATQIQNEDGSLVSLIGMSVEQRKALANQLLTPSSNVSTHTLNKKVY
RHIKNRDVVLMNRQPTLHKASMMGHKVRVLPNEKTLRLHYANTGAYNADF
DGDEMNMHFPQNENARAEALNLANTDSQYLTPTSGSPVRGLIQDHISAGV
WLTSKDSFFTREQYQQYIYGCIRPEDGHTTRSKIVTLPPTIFKPYPLWTG
KQIITTVLLNVTPPDMPGINLISKNKIKNEYWGKGSLENEVLFKDGALLC
GILDKSQYGASKYGIVHSLHEVYGPEVAAKVLSVLGRLFTNYITATAFTC
GMDDLRLTAEGNKWRTDILKTSVDTGREAAAEVTNLDKDTPADDPELLKR
LQEILRDNNKSGILDAVTSSKVNAITSQVVSKCVPDGTMKKFPCNSMQAM
ALSGAKGSNVNVSQIMCLLGQQALEGRRVPVMVSGKTLPSFKPYETDAMA
GGYVKGRFYSGIKPQEYYFHCMAGREGLIDTAVKTSRSGYLQRCLTKQLE
GVHVSYDNSIRDADGTLVQFMYGGDAIDITKESHMTQFEFCLDNYYALLK
KYNPSALIEHLDVESALKYSKKTLKYRKKHSPHYKQSVKYDPVLAKYNPA
KYLGSVSENFQDKLESFLDKNSKLFKSSDGVNEKKFRALMQLKYMRSLIN
PGEAVGIIASQSVGEPSTQMNVTLGIPRLREIVMTASAAIKTPQMTLPIW
NDVSDEQADTFCKSISKVLLSEVIDKVIVTETTAARSYVIHMRFFDNNEY
SEEYDVSKEELQNVISNQFIHLLEAAIVKEIKKQKNMNKVQRDRQSAIIS
HHRFITKYNFDDESGKWCEFKLELAADTEKLLMVNIVEEICRKSIIRQIP
HIDRCVHPEPENGKRVLVTEGVNFQAMWDQEAFIDVDGITSNDVAAVLKT
YGVEAARNTIVNEINNVFSRYAISVSFRHLDLIADMMTRQGTYLAFNRQG
METSTSSFMKMSYETTCQFLTKAVLDNEREQLDSPSARIVVGKLNNVGTG
SFDVLAKVPNA
3D structure
PDB5w66 Structural mechanism of ATP-independent transcription initiation by RNA polymerase I.
ChainA
Resolution3.9 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna A D629 D631 D551 D553
BS02 dna A R475 S1014 Y1018 E1616 T1617 Q1620 R397 S936 Y940 E1414 T1415 Q1418
BS03 ZN A C102 C105 C233 C236 C102 C105 C203 C206
BS04 ZN A C62 C65 H75 C62 C65 H75
Gene Ontology
Molecular Function
GO:0001054 RNA polymerase I activity
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
GO:1990841 promoter-specific chromatin binding
Biological Process
GO:0006351 DNA-templated transcription
GO:0006360 transcription by RNA polymerase I
GO:0006361 transcription initiation at RNA polymerase I promoter
GO:0006362 transcription elongation by RNA polymerase I
GO:0006363 termination of RNA polymerase I transcription
GO:0042254 ribosome biogenesis
GO:0042790 nucleolar large rRNA transcription by RNA polymerase I
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0005634 nucleus
GO:0005730 nucleolus
GO:0005736 RNA polymerase I complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:5w66, PDBe:5w66, PDBj:5w66
PDBsum5w66
PubMed28623663
UniProtP10964|RPA1_YEAST DNA-directed RNA polymerase I subunit RPA190 (Gene Name=RPA190)

[Back to BioLiP]