Structure of PDB 5vj7 Chain A

Receptor sequence

>5vj7A (length=462) Species: 1185654 (Pyrococcus furiosus COM1)

IKERVPTPERSVGERVRDFGEVNLGYSWELALREAERCLQCPVEYAPCIK
GCPVHINIPGFIKALRENRDNPSKAVREALRIIWRDNTLPAITGRVCPQE
EQCEGACVVGKVGDPINIGKLERFVADYAREHGIDDELLLEEIKGIKRNG
KKVAIIGAGPAGLTCAADLAKMGYEVTIYEALHQPGGVLTYGIPEFRLPK
EILRKELKKLSLLGVEIKTDHIVGKTITIQELLQEYDAVFIGTGAGTPKL
PNIPGINLNGIYSANEFLTRINLMKAYKFPEYDTPIVVGKKVVVIGAGNT
AMDAARSALRLGAEVTIAYRRGREDMTARIEEVKHAEEEGVKFMFFVNPV
EFIGDENGNVKAVKFEKMKPGKRKIVGTGEYITVEADTVIIAIGQTPNKV
LWRATPELKVDEWGRIVVDENLMTSIPGVFAGGDAIRGEATVILAMGDGR
KAAKAIHQYLSK

3D structure

• PDB: 5vj7 Two functionally distinct NADP(+)-dependent ferredoxin oxidoreductases maintain the primary redox balance of Pyrococcus furiosus.
• Chain: A
• Resolution: 2.55 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): P164 I197 P198 F200 D307 I454 M457 G458
• Catalytic site (residue number reindexed from 1): P160 I193 P194 F196 D303 I443 M446 G447

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	A	C56 I60 C101 Q103 Q106 C107 I122 E126 I454	C52 I56 C97 Q99 Q102 C103 I118 E122 I443
BS02	SF4	A	C42 L43 C45 C52 C111 V112 I120	C38 L39 C41 C48 C107 V108 I116
BS03	FAD	A	V100 G161 G163 P164 E184 A185 L186 G191 V192 I197 R201 V227 T247 G248 N303 T304 D445 T452 V453 A456	V96 G157 G159 P160 E180 A181 L182 G187 V188 I193 R197 V223 T243 G244 N299 T300 D434 T441 V442 A445

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

External links

• PDB: RCSB:5vj7, PDBe:5vj7, PDBj:5vj7
• PDBsum: 5vj7
• PubMed: 28705933
• UniProt: I6V148