Structure of PDB 5vc7 Chain A

Receptor sequence

>5vc7A (length=544) Species: 273075 (Thermoplasma acidophilum DSM 1728)

EVSRISYEDIGGLSEQLGKIREMIELPLKHPELFERLGITPPKGVILYGP
PGTGKTLIARAVANESGANFLSINGPEIMSKYYGQSEQKLREIFSKAEET
APSIIFIDEIDSIAPKREEVQGEVERRVVAQLLTLMDGMKERGHVIVIGA
TNRIDAIDPALRRPGRFDREIEIGVPDRNGRKEILMIHTRNMPLGMSEEE
KNKFLEEMADYTYGFVGADLAALVRESAMNALRRYLPEIDLDKPIPTEIL
EKMVVTEDDFKNALKSIEPSSLREVMVEVPNVHWDDIGGLEDVKREIKET
VELPLLKPDVFKRLGIRPSKGFLLYGPPGVGKTLLAKAVATESNANFISI
KGPEVLSKWVGESEKAIREIFKKAKQVAPAIVFLDEIDSIAPRRGTTSDS
GVTERIVNQLLTSLDGIEVMNGVVVIGATNRPDIMDPALLRAGRFDKLIY
IPPPDKEARLSILKVHTKNMPLAPDVDLNDIAQRTEGYVGADLENLCREA
GMNAYRENPDATSVSQKNFLDALKTIRPSVDEEVIKFYRTLSET

3D structure

• PDB: 5vc7 Structure of a AAA+ unfoldase in the process of unfolding substrate.
• Chain: A
• Resolution: 3.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K237 T238 D290 N334 K443 K514 T515 D567 E568 D597 A673
• Catalytic site (residue number reindexed from 1): K55 T56 D108 N152 K261 K332 T333 D385 E386 D415 A491

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	A	D191 I192 G193 G234 T235 G236 K237 T238 L239 I366 H370 G399	D9 I10 G11 G52 T53 G54 K55 T56 L57 I184 H188 G217
BS02	ATP	A	D468 I469 G470 G511 V512 G513 K514 T515 L516 I644 E676	D286 I287 G288 G329 V330 G331 K332 T333 L334 I462 E494

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity

External links

• PDB: RCSB:5vc7, PDBe:5vc7, PDBj:5vc7
• PDBsum: 5vc7
• PubMed: 28390173
• UniProt: O05209|VAT_THEAC VCP-like ATPase (Gene Name=vat)