Structure of PDB 5uv1 Chain A

Receptor sequence

>5uv1A (length=508) Species: 2711 (Citrus sinensis)

SIWDHDFLQSLNSNYTDETYKRRAEELKGKVKTAIKDVTEPLDQLELIDN
LQRLGLAYHFEPEIRNILRNIHNHNKDYNWRKENLYATSLEFRLLRQHGY
PVSQEVFSGFKDDKVGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTS
KHLKEMMIDVFVAEQAKRALELPLHWKAPMLEARWFIHVYEKREDKNHLL
LELAKLEFNTLQAIYQEELKDISGWWKDTGLGEKLSFARNRLVASFLWSM
GIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTLDELEIFTDAVARWDI
NYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDMLLSIKHAWLGLIQ
AYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIITISYLSGTNPIIKKEL
EFLESNPDIVHWSSKIFRLQDDLGTSSDVPKSIQCYMHETGASEEVAREH
IKDMMRQMWKKVNAYTADKDSPLTRTTAEFLLNLVRMSHFMYLHGDVGFT
LLFQPIPL

3D structure

• PDB: 5uv1 Structural Characterization of Early Michaelis Complexes in the Reaction Catalyzed by (+)-Limonene Synthase from Citrus sinensis Using Fluorinated Substrate Analogues.
• Chain: A
• Resolution: 2.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R306 W315 I336 D343 D347 S444 I445 T446 R485 D488 T492 Y565
• Catalytic site (residue number reindexed from 1): R239 W248 I269 D276 D280 S377 I378 T379 R418 D421 T425 Y492
• Enzyme Commision number: 4.2.3.20: (R)-limonene synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MN	A	D343 D347	D276 D280
BS02	MN	A	D343 D347	D276 D280
BS03	0FV	A	W315 I336 D343 T446 F484 R485 D488	W248 I269 D276 T379 F417 R418 D421	MOAD: Ki=2.4uM PDBbind-CN: -logKd/Ki=5.62,Ki=2.4uM

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0010333 terpene synthase activity
• GO:0016829 lyase activity
• GO:0034002 (R)-limonene synthase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0016102 diterpenoid biosynthetic process

Cellular Component

• GO:0009507 chloroplast

External links

• PDB: RCSB:5uv1, PDBe:5uv1, PDBj:5uv1
• PDBsum: 5uv1
• PubMed: 28272876
• UniProt: A0A1C9J6A7|RLC1_CITSI (R)-limonene synthase 1, chloroplastic