Structure of PDB 5urk Chain A

Receptor sequence

>5urkA (length=1706) Species: 9606 (Homo sapiens)

LAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPALKP
KPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLC
APTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMV
GSFGKRLATYGITVAELTGKEEISATQIIVCTPEKWDIITRKGGERTYTQ
LVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPN
YEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEI
VYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSA
STEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQ
VLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAG
RPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVL
GNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLV
HTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKP
TLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSA
KINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQL
TDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLN
HNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPD
FQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVP
VFEPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPL
PVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKT
ICAEFAILRMLLQSSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVL
LTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVH
LIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCS
ATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKK
PVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDS
TLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVA
AHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQ
GSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTW
TFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDE
MDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYEN
IPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAE
LQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKD
SYLKQLPHFTSEHIKRCTESVFDIERNALLQLTDSQIADVARFCNRYPNI
ELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVV
IGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCD
QEYKFS

3D structure

• PDB: 5urk Discovery of Allosteric Inhibitors Targeting the Spliceosomal RNA Helicase Brr2.
• Chain: A
• Resolution: 2.95 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	8LP	A	F1254 F1255 P1257 D1678 P1680 I1681 K1710 F1713 P1723	F847 F848 P850 D1271 P1273 I1274 K1303 F1306 P1316	PDBbind-CN: -logKd/Ki=6.10,IC50=0.79uM BindingDB: IC50=790nM

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:5urk, PDBe:5urk, PDBj:5urk
• PDBsum: 5urk
• PubMed: 28586220
• UniProt: O75643|U520_HUMAN U5 small nuclear ribonucleoprotein 200 kDa helicase (Gene Name=SNRNP200)