Structure of PDB 5urj Chain A

Receptor sequence

>5urjA (length=1707) Species: 9606 (Homo sapiens)

LAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPALKP
KPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLC
APTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMV
GSFGKRLATYGITVAELTGEISATQIIVCTPEKWDIITRKGGERTYTQLV
RLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYE
DVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVY
EKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSAST
EVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVL
VSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRP
QYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGN
VQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHT
AALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTL
SEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKI
NVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTD
KTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHN
EIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPDFQ
WDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVF
EPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPV
SALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTIC
AEFAILRMLLQSSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVLLT
GETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLI
GGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSAT
STFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPV
IVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTL
KETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAH
LVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGS
KKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTF
LYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMD
VAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIP
IRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQ
SDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSY
LKQLPHFTSEHIKRCTDESVFDIDEERNALLQLTDSQIADVARFCNRYPN
IELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWV
VIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGC
DQEYKFS

3D structure

• PDB: 5urj Discovery of Allosteric Inhibitors Targeting the Spliceosomal RNA Helicase Brr2.
• Chain: A
• Resolution: 2.75 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	8LS	A	H1235 F1255 V1256 P1257 D1678 P1680 I1681 S1709 K1710 D1712 F1713 K1716 P1723	H826 F846 V847 P848 D1269 P1271 I1272 S1300 K1301 D1303 F1304 K1307 P1314	PDBbind-CN: -logKd/Ki=5.28,IC50=5.3uM

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding

External links

• PDB: RCSB:5urj, PDBe:5urj, PDBj:5urj
• PDBsum: 5urj
• PubMed: 28586220
• UniProt: O75643|U520_HUMAN U5 small nuclear ribonucleoprotein 200 kDa helicase (Gene Name=SNRNP200)