Structure of PDB 5uqu Chain A

Receptor sequence

>5uquA (length=438) Species: 451804 (Aspergillus fumigatus A1163)

GSTAEPDLKTALKAVIPAKRELFKQVKERSDEVIGEVKVANVIGGMRGLK
SMLWEGSVLDPEEGIRFHGKTIKDCQKELPKGTSGTEMLPEAMFWLLLTG
QVPSTNQVRAFSRELAEQSHLPQHILDLIKSFPRSMHPMTQLSIAVAALN
TESKFAKAYEKGLSKADYWEPTFDDSISLLAKIPRVAALVFRPDEVDQVG
TQALDASQDWSYNFAELLGKGGKENQDFHDLLRLYLALHGDHEGGNVSAH
ATHLVGSALSDPFLSYSAGLLGLAGPLHGLAAQEVLRWILAMQDKIGTKF
TDDDVRNYLWDTLKSGRVVPGYGHAVLRKPDPRFQALMDFAATRPDVLAN
PVFQLVKKNSEIAPAVLTEHGKTKNPHPNVDAASGVLFYHYGFQQPLYYT
VTFGVSRALGPLVQLIWDRALGLPIERPKSINLLGLKK

3D structure

• PDB: 5uqu Comparative studies of Aspergillus fumigatus 2-methylcitrate synthase and human citrate synthase.
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S275 H305 H351 R360 D408
• Catalytic site (residue number reindexed from 1): S248 H278 H324 R333 D381
• Enzyme Commision number: 2.3.3.16: citrate synthase (unknown stereospecificity).
  2.3.3.5: 2-methylcitrate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	OAA	A	H269 N273 H305 H351 R360 R434	H242 N246 H278 H324 R333 R407

Gene Ontology

Molecular Function

• GO:0004108 citrate (Si)-synthase activity
• GO:0016740 transferase activity
• GO:0036440 citrate synthase activity
• GO:0042802 identical protein binding
• GO:0046912 acyltransferase activity, acyl groups converted into alkyl on transfer
• GO:0050440 2-methylcitrate synthase activity

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0006099 tricarboxylic acid cycle
• GO:0019629 propionate catabolic process, 2-methylcitrate cycle

Cellular Component

• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix

External links

• PDB: RCSB:5uqu, PDBe:5uqu, PDBj:5uqu
• PDBsum: 5uqu
• PubMed: 31141475
• UniProt: B0YD89|PRPC_ASPFC 2-methylcitrate synthase, mitochondrial (Gene Name=mcsA)