Structure of PDB 5upi Chain A

Receptor sequence

>5upiA (length=752) Species: 272558 (Halalkalibacterium halodurans C-125)

HAVSVGKGSYATEFPEIDFGGINDPGFRDQQGEPPATIYRSDRVTGPMQT
NSWWGSLAVDRFSMNQYPHPFSVRHRAEGLHVFYDAPHNMVVHENREAGT
WHIHGAIGTDFTIKHSGTANFEQAVVDDYNDWYVRGLLENGAHQMAITYG
VGSPYIFVEYEDGSAVLDFDIAPDVWEMNGHVIGFSTHDHKHYAAFAPPG
QNWSGIGSKTLTNNADYIAIAKLPEKDGNMLAKFEQYAYSVVRDAVADWT
YDEATGTVTTTFEVTTEAKVQGAPDGTIFALYPHQYRHLASSSENQLLQN
YQYEIIRGTMIGLEGKRFTTELTYPGVLPSLPDLGDYDRERLIGYLHDAT
SDYPTGSDTYELGKYIGKLATLAPIADQMGEYELAEQFRGELKDILEDWL
QATNASGQLKGKNLFYYNENWGTILGYHAAHSSATRINDHHFHYGYFVKA
AAEIARADQEWAKSENWGGMIDLLIRDFMADRDDDLFPYLRMFDPYSGNS
WADGLATFDAGNNQQSSSEAMHAWTNVILWAEATGNKALRDRAIYLYTTE
MSAINEYFFDVHQEIFPEEYGPEIVTINWGGKMDHATWWNSGKVEKYAIN
WLPFHGGSLYLGHHPDYVDRAYEELRRDIGSTDWNLWSNLVWMYRAFTNP
DDALQQMEASIDDYGLFDPGNEKIIERGSTKAQTYHWIHNLAELGRVDPT
VTANHPIYAVFNKNGNRTYIVYNFSDSPITVQFSDGHSIQVEPHSFNIGN
GD

3D structure

• PDB: 5upi The quaternary structure of beta-1,3-glucan contributes to its recognition and hydrolysis by a multimodular family 81 glycoside hydrolase
• Chain: A
• Resolution: 1.65 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.39: glucan endo-1,3-beta-D-glucosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	A	R463 F535 W615	R436 F508 W588
BS02	BGC	A	H458 N465 D466 D530 N540 W615	H431 N438 D439 D503 N513 W588
BS03	BGC	A	Y387 K391 H458 W616 R704	Y360 K364 H431 W589 R677
BS04	BGC	A	W616 E699 R704	W589 E672 R677

Gene Ontology

Molecular Function

• GO:0052861 endo-1,3(4)-beta-glucanase activity

External links

• PDB: RCSB:5upi, PDBe:5upi, PDBj:5upi
• PDBsum: 5upi
• UniProt: Q9KG76|ENG1_HALH5 Glucan endo-1,3-beta-D-glucosidase (Gene Name=BH0236)