Structure of PDB 5upg Chain A

Receptor sequence

>5upgA (length=300) Species: 208964 (Pseudomonas aeruginosa PAO1)

SMIKQRTLKNIIRATGVGLHSGEKVYLTLKPAPVDTGIVFCRTDLDPVVE
IPARAENVGETTMSTTLVKGDVKVDTVEHLLSAMAGLGIDNAYVELSASE
VPIMDGSAGPFVFLIQSAGLQEQEAAKKFIRIKREVSVEEGDKRAVFVPF
DGFKVSFEIDFDHPVFRGRTQQASVDFSSTSFVKEVSRARTFGFMRDIEY
LRSQNLALGGSVENAIVVDENRVLNEDGLRYEDEFVKHKILDAIGDLYLL
GNSLIGEFRGFKSGHALNNQLLRTLIADKDAWEVVTFEDARTAPISYMRP

3D structure

• PDB: 5upg Crystal structure of LpxC from Pseudomonas aeruginosa in complex with PF-5081090
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Enzyme Commision number: 3.5.1.108: UDP-3-O-acyl-N-acetylglucosamine deacetylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	8GJ	A	L18 M62 E77 T190 F191 G192 F193 I197 L200 H237 D241 H264	L19 M63 E78 T191 F192 G193 F194 I198 L201 H238 D242 H265	BindingDB: IC50=1.1nM
BS02	8GJ	A	A84 F152 F176 S177 T179 S180 F181 S252 L253 I294 S295 Y296 M297	A85 F153 F177 S178 T180 S181 F182 S253 L254 I295 S296 Y297 M298	BindingDB: IC50=1.1nM
BS03	ZN	A	H78 H237 D241	H79 H238 D242
BS04	ZN	A	D277 D279	D278 D280

Gene Ontology

Molecular Function

• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding
• GO:0103117 UDP-3-O-acyl-N-acetylglucosamine deacetylase activity

Biological Process

• GO:0006796 phosphate-containing compound metabolic process
• GO:0009245 lipid A biosynthetic process
• GO:0019637 organophosphate metabolic process
• GO:1901135 carbohydrate derivative metabolic process

External links

• PDB: RCSB:5upg, PDBe:5upg, PDBj:5upg
• PDBsum: 5upg
• UniProt: P47205|LPXC_PSEAE UDP-3-O-acyl-N-acetylglucosamine deacetylase (Gene Name=lpxC)