Structure of PDB 5ul4 Chain A

Receptor sequence

>5ul4A (length=729) Species: 1404 (Priestia megaterium)

LSTKSIEYYLKELKEIFSQIWLKPSEIEKRCEELFKRSKEFDYKRILVSG
ETDNTTLYVIEDSSKIHVFSPNRDLRENPLLMRWHPSWYEIESKEIYYKC
FLSCEELYEHLELPTVTLVNLCVIENFPIPRLNLSTGTLSSYLRKEQLAK
VELIDMQVGTTINQIIKNLLDSQPDIIGLSVNFGQKKLAFEILDLIYSHI
ENGDLSSIITVGNVIPSFSPEQFFERYPSLLICDKEGEYTLRDLIKMLKK
ELKLDEVNGISYVDESGEVKHNVAETVNFKEEVPTPSLDILGEISKFRGA
LTLETSRGCDYSRCTFCPRDHKLRSWRPLSVEQTLKQLDDILRAGKHFNI
KPHIYMADEEFIGELPNGTEAQRIIDICEGLLKREEKIKFDFAARADSVY
EPKRTKEWNVERLKMWHYCALAGADRIFIGVESGSNQQLKRYGKGTTSEQ
NIIALRLVSALGINLRIGFIMFDQLMKGLDNLKENLDFLERTDALMKPID
IGDMTYEELYDKLLNDKEFIEKHKTGKPVYTIVSYMLASMEILMNTPYSR
MVQLTERKEEVNLIMNDGKPDMNMGRYATSFVDKTNGNLSEACQMWIDSN
FGVMYTIKSLHKVANPREKKKLYSYMETHREISHFLLKYLVYNLSPDKES
QIILSDFLRMHSMEHILDNVGDGSKENILNVMTNWQLIMEKLLRDVEADL
NKGIITDSEDHRLHNTLKRWFSDMGNWSL

3D structure

• PDB: 5ul4 A B12-dependent radical SAM enzyme involved in oxetanocin A biosynthesis.
• Chain: A
• Resolution: 1.85 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	A	C313 S316 C318 C321 R399	C309 S312 C314 C317 R395
BS02	B12	A	F131 R135 S139 L143 G182 L183 S184 F187 G216 N217 C237 K239 E240 G241 E242 E308 R311 P322 H325 K326 A361 E363	F127 R131 S135 L139 G178 L179 S180 F183 G212 N213 C233 K235 E236 G237 E238 E304 R307 P318 H321 K322 A357 E359
BS03	SAM	A	F432 E436 G472 I474 M544 E545	F428 E432 G468 I470 M540 E541

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0031419 cobalamin binding
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

External links

• PDB: RCSB:5ul4, PDBe:5ul4, PDBj:5ul4
• PDBsum: 5ul4
• PubMed: 28346939
• UniProt: O24770