Structure of PDB 5ugh Chain A

Receptor sequence
>5ughA (length=369) Species: 9606 (Homo sapiens) [Search protein sequence]
FIEEGTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAK
TGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALE
QQSEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLP
WLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKE
KVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPDAGLTGRKIIVDTYGGWGA
HGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSH
PLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAA
YGHFGRDSFPWEVPKKLKY
3D structure
PDB5ugh Targeting S-adenosylmethionine biosynthesis with a novel allosteric inhibitor of Mat2A.
ChainA
Resolution2.062 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) H29 D31 K32 E57 E70 K181 F250 D258 A259 R264 K265 K285 K289 D291
Catalytic site (residue number reindexed from 1) H18 D20 K21 E46 E59 K157 F226 D232 A233 R238 K239 K259 K263 D265
Enzyme Commision number 2.5.1.6: methionine adenosyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 8AJ A L315 S331 I332 F333 E342 L289 S305 I306 F307 E316 MOAD: Kd=170nM
PDBbind-CN: -logKd/Ki=6.77,Kd=170nM
BS02 8AJ A F18 F20 Q190 G273 W274 F7 F9 Q166 G247 W248 MOAD: Kd=170nM
PDBbind-CN: -logKd/Ki=6.77,Kd=170nM
Gene Ontology
Molecular Function
GO:0004478 methionine adenosyltransferase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016740 transferase activity
GO:0036094 small molecule binding
GO:0042802 identical protein binding
GO:0046872 metal ion binding
Biological Process
GO:0006556 S-adenosylmethionine biosynthetic process
GO:0006730 one-carbon metabolic process
GO:0034214 protein hexamerization
GO:0051291 protein heterooligomerization
GO:0061431 cellular response to methionine
GO:1904263 positive regulation of TORC1 signaling
GO:1990830 cellular response to leukemia inhibitory factor
Cellular Component
GO:0005829 cytosol
GO:0048269 methionine adenosyltransferase complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:5ugh, PDBe:5ugh, PDBj:5ugh
PDBsum5ugh
PubMed28553945
UniProtP31153|METK2_HUMAN S-adenosylmethionine synthase isoform type-2 (Gene Name=MAT2A)

[Back to BioLiP]