Structure of PDB 5u30 Chain A

Receptor sequence

>5u30A (length=1085) Species: 1356854 (Alicyclobacillus acidoterrestris ATCC 49025)

SMAVKSIKVKLRLDDMPEIRAGLWKLHKEVNAGVRYYTEWLSLLRQENLY
RRSPNGDGEQECDKTAEECKAELLERLRARQVENGHRGPAGSDDELLQLA
RQLYELLVPQAIGAKGDAQQIARKFLSPLADKDAVGGLGIAKAGNKPRWV
RMREAGEWEEEKEKAETRKSADRTADVLRALADFGLKPLMRVYTDSEMSS
VEWKPLRKGQAVRTWDRDMFQQAIERMMSWESWNQRVGQEYAKLVEQKNR
FEQKNFVGQEHLVHLVNQLQQDMKEASPGLESKEQTAHYVTGRALRGSDK
VFEKWGKLAPDAPFDLYDAEIKNVQRRNTRRFGSHDLFAKLAEPEYQALW
REDASFLTRYAVYNSILRKLNHAKMFATFTLPDATAHPIWTRFDKLGGNL
HQYTFLFNEFGERRHAIRFHKLLKVENGVAREVDDVTVPISMSEQLDNLL
PRDPNEPIALYFRDYGAEQHFTGEFGGAKIQCRRDQLAHMHRRRRDVYLN
VSVRVQSQSEARGERRPPYAAVFRLVGDNHRAFVHFDKLSDYLAEHPDDG
KLGSEGLLSGLRVMSVALGLRTSASISVFRVARKDELKPNSKGRVPFFFP
IKGNDNLVAVHERSQLLKLPGETESKDLRAIREERQRTLRQLRTQLAYLR
LLVRCGSEDVGRRERSWAKLIEQPVDAANHMTPDWREAFENELQKLKSLH
GICSDKEWMDAVYESVRRVWRHMGKQVRDWRKDVRSGERPKIRGYAKDVV
GGNSIEQIEYLERQYKFLKSWSFFGKVSGQVIRAEKGSRFAITLREHIDH
AKEDRLKKLADRIIMEALGYVYALDERGKGKWVAKYPPCQLILLAELSEY
QFNNDRPPSENNQLMQWSHRGVFQELINQAQVHDLLVGTMYAAFSSRFDA
RTGAPGIRCRRVPARCTQEHNPEPFPWWLNKFVVEHTLDACPLRADDLIP
TGEGEIFVSPFSAEEGDFHQIHAALNAAQNLQQRLWSDFDISQIRLRCDW
GEVDGELVLIPRLTGKRTADSYSNKVFYTNTGVTYYERERGREKSVVLMR
DPSGIINRGNWTRQKEFWSMVNQRIEGYLVKQIRS

3D structure

• PDB: 5u30 PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease.
• Chain: A
• Resolution: 2.92 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	V3 K4 S5 K9 W234 V238 P279 R294 K323 Q326 R327 H336 D337 K370 A374 K375 T379 T381 H388 P389 W391 R415 S442 M443 S444 Q446 Q482 R484 R485 H490 H492 Y501 N503 R597 F600 H614 S617 Q618 D630 I634 R653 R657 R665 W723 R724 G727 R731 V737 R738 S739 R742 P743 K744 I745 R746 G747 V753 G754 G755 Q760 L764 Q767 K769 K772 S773 W774 S775 F776 G782 Q783 V784 I785 R792 F793 A794 I795 T796 L797 H800 H803 D807 K810 K811 D814 E819 V824 Y825 K832 W835 Y839 F855 N856 N857 P861 N864 N865 M868 N881 R904 R914 A917 R918 D949 H972 Q973 L978	V4 K5 S6 K10 W233 V237 P278 R293 K322 Q325 R326 H335 D336 K369 A373 K374 T378 T380 H387 P388 W390 R414 S441 M442 S443 Q445 Q481 R483 R484 H489 H491 Y498 N500 R594 F597 H611 S614 Q615 D627 I631 R650 R654 R662 W720 R721 G724 R728 V734 R735 S736 R739 P740 K741 I742 R743 G744 V750 G751 G752 Q757 L761 Q764 K766 K769 S770 W771 S772 F773 G779 Q780 V781 I782 R789 F790 A791 I792 T793 L794 H797 H800 D804 K807 K808 D811 E816 V821 Y822 K829 W832 Y836 F852 N853 N854 P858 N861 N862 M865 N878 R901 R911 A914 R915 D946 H969 Q970 L975
BS02	dna	A	V3 A117 Q118 A142 G143 N144 Q222 E226 S230 S233 W234 R237 G280 L281 K284 E285 A288 H289 R294 R297 T330 R331 R332 S335 K396 N400 G478 S505 R507 L573 R574 R643 R646 R786 E788 K789 R792 F793 R798 K805 L850 E852 Y853 F855 R859 P861 S862 Q866 M868 W870 S871 R873 G874 A896 F897 S899 R900 R911 W930 E956 W1097	V4 A118 Q119 A143 G144 N145 Q221 E225 S229 S232 W233 R236 G279 L280 K283 E284 A287 H288 R293 R296 T329 R330 R331 S334 K395 N399 G477 S502 R504 L570 R571 R640 R643 R783 E785 K786 R789 F790 R795 K802 L847 E849 Y850 F852 R856 P858 S859 Q863 M865 W867 S868 R870 G871 A893 F894 S896 R897 R908 W927 E953 W1068
BS03	dna	A	Q119 R122 A133 V134 G135 G136 A140 A142 G143 N144 R147 Q211 V213 R218	Q120 R123 A134 V135 G136 G137 A141 A143 G144 N145 R148 Q210 V212 R217

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:5u30, PDBe:5u30, PDBj:5u30
• PDBsum: 5u30
• PubMed: 27984729
• UniProt: T0D7A2|CS12B_ALIAG CRISPR-associated endonuclease Cas12b (Gene Name=cas12b)