Structure of PDB 5txz Chain A

Receptor sequence

>5txzA (length=328) Species: 9606 (Homo sapiens)

WMPAYACQRPTPLTHHNTGLSEALEILAEAAGFEGSEGRLLTFCRAASVL
KALPSPVTTLSQLQGLPHFGEHSSRVVQELLEHGVCEEVERVRRSERYQT
MKLFTQIFGVGVKTADRWYREGLRTLDDLREQPQKLTQQQKAGLQHHQDL
STPVLRSDVDALQQVVEEAVGQALPGATVTLTGGFRRGKLQGHDVDFLIT
HPKEGQEAGLLPRVMCRLQDQGLILYHQHAFERSFCIFRLPQPGSWKAVR
VDLVVAPVSQFPFALLGWTGSKLFQRELRRFSRKEKGLWLNSHGLFDPEQ
KTFFQAASEEDIFRHLGLEYLPPEQRNA

3D structure

• PDB: 5txz Time-lapse crystallography snapshots of a double-strand break repair polymerase in action.
• Chain: A
• Resolution: 1.651 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D330 D332 D418
• Catalytic site (residue number reindexed from 1): D194 D196 D252
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	L177 F385 E386 R387 R442 R445 R449 K450 L456 N457 H459	L41 F231 E232 R233 R276 R279 R283 K284 L290 N291 H293
BS02	dna	A	G245 V246 G247 K249 T250 D330 D332 F389 R416 D418 G433 W434 T435 G436 K438	G109 V110 G111 K113 T114 D194 D196 F235 R250 D252 G267 W268 T269 G270 K272
BS03	dna	A	G174 R175 T178 F179 H204 G206 H208 S209	G38 R39 T42 F43 H68 G70 H72 S73
BS04	TTP	A	G319 G320 R323 H329 D330 D332 G433 G436 K438	G183 G184 R187 H193 D194 D196 G267 G270 K272
BS05	MG	A	D330 D332	D194 D196
BS06	MG	A	D330 D332 D418	D194 D196 D252
BS07	CA	A	D330 D332 D418	D194 D196 D252

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

Cellular Component

• GO:0005634 nucleus

External links

• PDB: RCSB:5txz, PDBe:5txz, PDBj:5txz
• PDBsum: 5txz
• PubMed: 28811466
• UniProt: Q9NP87|DPOLM_HUMAN DNA-directed DNA/RNA polymerase mu (Gene Name=POLM)