Structure of PDB 5tr4 Chain A

Receptor sequence
>5tr4A (length=972) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
IDESLYSRQLYVLGKEAMLKMQTSNVLILGLKGLGVEIAKNVVLAGVKSM
TVFDPEPVQLADLSTQFFLTEKDIGQKRGDVTRAKLAELNAYVPVNVLDS
LDDVTQLSQFQVVVATDTVSLEDKVKINEFCHSSGIRFISSETRGLFGNT
FVDLGDEFTVLDPTGEEPRTGMVSDIEPDGTVTMLDDNRHGLEDGNFVRF
SEVEGLDKLNDGTLFKVEVLGPFAFRIGSVKEYGEYKKGGIFTEVKVPRK
ISFKSLKQQLSNPEFVFSDFAKFDRAAQLHLGFQALHQFAVRHNGELPRT
MNDEDANELIKLVTDLSVQQPEVLGEGVDVNEDLIKELSYQARGDIPGVV
AFFGGLVAQEVLKACSGKFTPLKQFMYFDSLESLPDPKNFPRNEKTTQPV
NSRYDNQIAVFGLDFQKKIANSKVFLVGSGAIGCEMLKNWALLGLGSGSD
GYIVVTDMDSIEKSNLNRQFLFRPKDVGKNKSEVAAEAVCAMNPDLKGKI
NAKIDRVGPETEEIFNDSFWESLDFVTNALDNVDARTYVDRRCVFYRKPL
LESGTLGTKGNTQVIIPRLTESYSSSRDPPEKSIPLCTLRSFPNKIDHTI
AWAKSLFQGYFTDSAENVNMYLTQPNFVKGVLESISDSLSKPHNFEDCIK
WARLEFEKKFNHDIKQLLFNFPKDAKTGEPFWSGAKRAPTPLEFDIYNND
HFHFVVAGASLRAYNYGIKSDKPNVDEYKSVIDHMIIPEFTPNANLKIQV
NDLVSSLPDPSTLAGFKLEPVDFEKDDDTNHHIEFITACSNCRAQNYFIE
TADRQKTKFIAGRIIPAIATTTSLVTGLVNLELYKLIDNKTDIEQYKNGF
VNLALPFFGFSEPIASPKGEYNNKKYDKIWDRFDIKGDIKLSDLIEHFEK
DEGLEITMLSYGVSLLYASFFPPKKLKERLNLPITQLVKLVTPAHVSTMI
LEICADDKEGEDVEVPFITIHL
3D structure
PDB5tr4 Identification and Characterization of a Small Molecule Inhibitor of the Ubiquitin Activating Enzyme (TAK-243)
ChainA
Resolution2.2 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R21 R481 D544 C600 T601 R603 N781 D782
Catalytic site (residue number reindexed from 1) R8 R468 D531 C587 T588 R590 N751 D752
Enzyme Commision number 6.2.1.45: E1 ubiquitin-activating enzyme.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 61T A A444 D470 M471 R481 K494 R519 V520 A542 L543 N545 D547 A548 Y551 A431 D457 M458 R468 K481 R506 V507 A529 L530 N532 D534 A535 Y538
Gene Ontology
Molecular Function
GO:0004839 ubiquitin activating enzyme activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008641 ubiquitin-like modifier activating enzyme activity
GO:0016874 ligase activity
Biological Process
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0006974 DNA damage response
GO:0016567 protein ubiquitination
GO:0036211 protein modification process
GO:0043412 macromolecule modification
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5tr4, PDBe:5tr4, PDBj:5tr4
PDBsum5tr4
PubMed
UniProtP22515|UBA1_YEAST Ubiquitin-activating enzyme E1 1 (Gene Name=UBA1)

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