Structure of PDB 5tpq Chain A

Receptor sequence
>5tpqA (length=446) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
ENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSE
ITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTASAASAT
AWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQAATPA
ALVAHVTSSKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGG
AKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLG
LFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAI
ELLSKNEKGFFLQVAGASIDAQDHAANPCGQIGETVDLDEAVQRALEFAK
KEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEE
DSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLKWSHP
3D structure
PDB5tpq Differential catalytic promiscuity of the alkaline phosphatase superfamily bimetallo core reveals mechanistic features underlying enzyme evolution.
ChainA
Resolution2.45 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D51 S102 A153 T155 S166 A322 D327 A328 H331 D369 H370 H412
Catalytic site (residue number reindexed from 1) D44 S95 A146 T148 S159 A315 D320 A321 H324 D362 H363 H405
Enzyme Commision number 3.1.3.1: alkaline phosphatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN A D51 S102 D369 H370 D44 S95 D362 H363
BS02 ZN A D327 H331 H412 D320 H324 H405
BS03 ZN A E134 H162 H452 E127 H155 H445
BS04 PO4 A S102 D327 H412 S95 D320 H405
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004035 alkaline phosphatase activity
GO:0004721 phosphoprotein phosphatase activity
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016791 phosphatase activity
GO:0030613 oxidoreductase activity, acting on phosphorus or arsenic in donors
GO:0033748 hydrogenase (acceptor) activity
GO:0046872 metal ion binding
Biological Process
GO:0006470 protein dephosphorylation
Cellular Component
GO:0030288 outer membrane-bounded periplasmic space
GO:0042597 periplasmic space

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Biological Process
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Cellular Component
External links
PDB RCSB:5tpq, PDBe:5tpq, PDBj:5tpq
PDBsum5tpq
PubMed29070681
UniProtP00634|PPB_ECOLI Alkaline phosphatase (Gene Name=phoA)

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