Structure of PDB 5tf9 Chain A

Receptor sequence
>5tf9A (length=262) Species: 9606 (Homo sapiens) [Search protein sequence]
KAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTK
SERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKCIVLVTELMTSGTLKTY
LKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTG
SVKIGDLGLATLKGTPEFMAPEMYAAAYDESVDVYAFGMCMLEMATSEYP
YSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIK
DLLNHAFFQEET
3D structure
PDB5tf9 Discovery and Characterization of Allosteric WNK Kinase Inhibitors.
ChainA
Resolution2.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D349 K351 D353 N354 D368 K375 T386
Catalytic site (residue number reindexed from 1) D137 K139 D141 N142 D156 K163 T165
Enzyme Commision number 2.7.11.1: non-specific serine/threonine protein kinase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 7AV A L252 F265 E268 V281 F283 L299 D368 L369 G370 A372 L43 F56 E59 V72 F74 L87 D156 L157 G158 A160 MOAD: Kd=4.4uM
BindingDB: IC50=420nM
BS02 ANP A G228 G230 K233 A248 M304 K351 G19 G21 K24 A39 M92 K139
BS03 MN A N354 D368 N142 D156
BS04 CA A S423 C425 S202 C204
Gene Ontology
Molecular Function
GO:0004672 protein kinase activity
GO:0005524 ATP binding
Biological Process
GO:0006468 protein phosphorylation

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Molecular Function
View graph for
Biological Process
External links
PDB RCSB:5tf9, PDBe:5tf9, PDBj:5tf9
PDBsum5tf9
PubMed27712055
UniProtQ9H4A3|WNK1_HUMAN Serine/threonine-protein kinase WNK1 (Gene Name=WNK1)

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