Structure of PDB 5t5t Chain A

Receptor sequence

>5t5tA (length=369) Species: 10116 (Rattus norvegicus)

GRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVV
GKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKN
GRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADF
GLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALL
CGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRAT
IKDIREHEWFKQDLPKYLFPEAKWHLGIRSQSRPNDIMAEVCRAIKQLDY
EWKVVNPYYLRVRRKNPVTSTFSKMSLQLYQVDSRTYLLDFRSIDDERPG
SHTIEFFEMCANLIKILAQ

3D structure

• PDB: 5t5t Selective Activation of AMPK beta 1-Containing Isoforms Improves Kidney Function in a Rat Model of Diabetic Nephropathy.
• Chain: A
• Resolution: 3.46 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D139 K141 E143 N144 D157 S176
• Catalytic site (residue number reindexed from 1): D131 K133 E135 N136 D149 S168
• Enzyme Commision number: 2.7.11.1: non-specific serine/threonine protein kinase.
  2.7.11.26: [tau protein] kinase.
  2.7.11.31: [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	STU	A	L22 G23 V24 G25 V30 A43 K45 M93 E94 Y95 V96 G99 E100 E143 N144 L146 D157	L14 G15 V16 G17 V22 A35 K37 M85 E86 Y87 V88 G91 E92 E135 N136 L138 D149
BS02	75O	A	V11 L18 K31 I46 D88	V3 L10 K23 I38 D80

Gene Ontology

Molecular Function

• GO:0004672 protein kinase activity
• GO:0004679 AMP-activated protein kinase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006468 protein phosphorylation

External links

• PDB: RCSB:5t5t, PDBe:5t5t, PDBj:5t5t
• PDBsum: 5t5t
• PubMed: 28289077
• UniProt: P54645|AAPK1_RAT 5'-AMP-activated protein kinase catalytic subunit alpha-1 (Gene Name=Prkaa1)