Structure of PDB 5t4g Chain A

Receptor sequence

>5t4gA (length=752) Species: 272558 (Halalkalibacterium halodurans C-125)

MHAVSVGKGSYATEFPEIDFGGINDPGFRDQQGEPPATIYRSDRVTGPMQ
TNSWWGSLAVDRFSMNQYPHPFSVRHRAEGLHVFYDAPHNMVVHENREAG
TWHIHGAIGTDFTIKHSGTANFEQAVVDDYNDWYVRGLLENGAHQMAITY
GVGSPYIFVEYEDGSAVLDFDIAPDVWEMNGHVIGFSTHDHKHYAAFAPP
GQNWSGIGSKTLTNNADYIAIAKLPEKDGNMLAKFEQYAYSVVRDAVADW
TYDEATGTVTTTFEVTTEAKVQGAPDGTIFALYPHQYRHLASSSENQLLQ
NYQYEIIRGTMIGLEGKRFTTELTYPGVLPSLPDLGDYDRERLIGYLHDA
TSDYPTGSDTYELGKYIGKLATLAPIADQMGEYELAEQFRGELKDILEDW
LQATNASGQLKGKNLFYYNENWGTILGYHAAHSSATRINDHHFHYGYFVK
AAAEIARADQEWAKSENWGGMIDLLIRDFMADRDDDLFPYLRMFDPYSGN
SWADGLATFDAGNNQESSSEAMHAWTNVILWAEATGNKALRDRAIYLYTT
EMSAINEYFFDVHQEIFPEEYGPEIVTINWGGKMDHATWWNSGKVEKYAI
NWLPFHGGSLYLGHHPDYVDRAYEELRRDIGSTDWNLWSNLVWMYRAFTN
PDDALQQMEASIDDYGLFDPGNEKIIERGSTKAQTYHWIHNLAELGRVDP
TVTANHPIYAVFNKNGNRTYIVYNFSDSPITVQFSDGHSIQVEPHSFNIG
NG

3D structure

• PDB: 5t4g Structural Analysis of a Family 81 Glycoside Hydrolase Implicates Its Recognition of beta-1,3-Glucan Quaternary Structure.
• Chain: A
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.39: glucan endo-1,3-beta-D-glucosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	Y387 H458 D466 F469 H470 E542 E546	Y361 H432 D440 F443 H444 E516 E520
BS02	BGC	A	Y387 K391 H458 W616 R704	Y361 K365 H432 W590 R678
BS03	BGC	A	E699 R704	E673 R678
BS04	BGC	A	R463 F535	R437 F509
BS05	BGC	A	H458 N465 D466 D530 N540 W615	H432 N439 D440 D504 N514 W589
BS06	BGC	A	F535 D536	F509 D510
BS07	BGC	A	F535 D536 A537 W606 K609	F509 D510 A511 W580 K583
BS08	BGC	A	W606 D611 T614 W615	W580 D585 T588 W589
BS09	BGC	A	T614 N617	T588 N591
BS10	BGC	A	G53 F54	G27 F28
BS11	BGC	A	F54 G383 S384	F28 G357 S358

Gene Ontology

Molecular Function

• GO:0052861 endo-1,3(4)-beta-glucanase activity

External links

• PDB: RCSB:5t4g, PDBe:5t4g, PDBj:5t4g
• PDBsum: 5t4g
• PubMed: 28781080
• UniProt: Q9KG76|ENG1_HALH5 Glucan endo-1,3-beta-D-glucosidase (Gene Name=BH0236)