Structure of PDB 5pzn Chain A

Receptor sequence

>5pznA (length=545) Species: 333284 (Hepatitis C virus (isolate Con1))

SMSYTWTGALITPCVYATTSRSASLRQKKVTFDRLQVLDDHYRDVLKEMK
AKASTVKAKLLSVEEACKLTPPHSARSKFGYGAKDVRNLSSKAVNHIRSV
WKDLLEDTETPIDTTIMAKNEVFCGRKPARLIVFPDLGVRVCEKMALYDV
VSTLPQAVMGSSYGFQYSPGQRVEFLVNAWKAKKCPMGFAYDTRCFDSTV
TENDIRVEESIYQCCDLAPEARQAIRSLTERLYIGGPLTNSKGQNCGYRR
CRASGVLTTSCGNTLTCYLKAAAACRAAKLQDCTMLVCGDDLVVICESAG
TQEDEASLRAFTEAMTRYSAPPGDPPKPEYDLELITSCSSNVSVAHDASG
KRVYYLTRDPTTPLARAAWETARHTPVNSWLGNIIMYAPTLWARMILMTH
FFSILLAQEQLEKALDCQIYGACYSIEPLDLPQIIQRLHGLSAFSLHSYS
PGEINRVASCLRKLGVPPLRVWRHRARSVRARLLSQGGRAATCGKYLFNW
AVRTKLKLTPIPAASQLDLSSWFVAGYSGGDIYHSLSRARPRWFM

3D structure

• PDB: 5pzn Discovery of a Hepatitis C Virus NS5B Replicase Palm Site Allosteric Inhibitor (BMS-929075) Advanced to Phase 1 Clinical Studies.
• Chain: A
• Resolution: 2.25 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.21.98: hepacivirin.
  3.4.22.-
  3.6.1.15: nucleoside-triphosphate phosphatase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	23E	A	A395 A396 H428 L492 G493 V494 P495 R498 V499 W500 R503	A367 A368 H400 L464 G465 V466 P467 R470 V471 W472 R475
BS02	8XP	A	F193 P197 R200 L204 L314 I363 S365 C366 S368 M414 Y448 Y452	F165 P169 R172 L176 L286 I335 S337 C338 S340 M386 Y420 Y424	PDBbind-CN: -logKd/Ki=7.39,IC50=0.041uM

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:5pzn, PDBe:5pzn, PDBj:5pzn
• PDBsum: 5pzn
• PubMed: 28430437
• UniProt: Q9WMX2|POLG_HCVCO Genome polyprotein