Structure of PDB 5o6e Chain A

Receptor sequence

>5o6eA (length=512) Species: 559292 (Saccharomyces cerevisiae S288C)

GLSKEQESIIKLAENGHNIFYTGSAGTGKSILLREMIKVLKGIYGRENVA
VTASTGLAACNIGGITIHSFAGIGLGKGDADKLYKKVRRSRKHLRRWENI
GALVVDEISMLDAELLDKLDFIARKIRKNHQPFGGIQLIFCGDFFQLPPV
SKDPNRPTKFAFESKAWKEGVKMTIMLQKVFRQRGDVKFIDMLNRMRLGN
IDDETEREFKKLSRPLPDDEIIPAELYSTRMEVERANNSRLSKLPGQVHI
FNAIDGGALEDEELKERLLQNFLAPKELHLKVGAQVMMVKNLDATLVNGS
LGKVIEFMDPETYFCYEALTNDPSMPPEKLETWAENPSKLKAAMEREQRK
SSVKEGFAKSDIGEPVSPLDSSVFDFMKRVVLENIKRKEQLMQTIHQNSA
GKRRLPLVRFKASDMSTRMVLVEPEDWAIEDENEKPLVSRVQLPLMLAWS
LSIHKSQGQTLPKVKVDLRRVFEKGQAYVALSRAVSREGLQVLNFDRTRI
KAHQKVIDFYLT

3D structure

• PDB: 5o6e Insights into the structural and mechanistic basis of multifunctional S. cerevisiae Pif1p helicase.
• Chain: A
• Resolution: 3.345 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.12: DNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T290 T301 S304 G309 L310 K312 V385 K387 R465 K525 N526 S703 H705 K706 R721 F723	T55 T66 S69 G74 L75 K77 V150 K152 R230 K290 N291 S452 H454 K455 R470 F472
BS02	dna	A	E498 R721	E263 R470
BS03	ADP	A	G261 G263 K264 S265 I266 F416 R417 T711	G26 G28 K29 S30 I31 F181 R182 T460
BS04	ALF	A	A260 K264 E342 Q381 R417 G709 R734	A25 K29 E107 Q146 R182 G458 R483

Gene Ontology

Molecular Function

• GO:0003678 DNA helicase activity

Biological Process

• GO:0000723 telomere maintenance
• GO:0006281 DNA repair

External links

• PDB: RCSB:5o6e, PDBe:5o6e, PDBj:5o6e
• PDBsum: 5o6e
• PubMed: 29202194
• UniProt: P07271|PIF1_YEAST ATP-dependent DNA helicase PIF1 (Gene Name=PIF1)