Structure of PDB 5o6b Chain A

Receptor sequence

>5o6bA (length=528) Species: 559292 (Saccharomyces cerevisiae S288C)

GLSKEQESIIKLAENGHNIFYTGSAGTGKSILLREMIKVLKGIYGRENVA
VTASTGLAACNIGGITIHSFAGIGLGKGDADKLYKKVRRSRKHLRRWENI
GALVVDEISMLDAELLDKLDFIARKIRKNHQPFGGIQLIFCGDFFQLPPV
SKPTKFAFESKAWKEGVKMTIMLQKVFRQRGDVKFIDMLNRMRLGNIDDE
TEREFKKLSRPLPDDEIIPAELYSTRMEVERANNSRLSKLPGQVHIFNAI
DGGALEDEELKERLLQNFLAPKELHLKVGAQVMMVKNLDATLVNGSLGKV
IEFMDPETYFCYEALTNDPSMPPEKLETWAENPSKLKAAMEREQSAVASR
KSSVKEGFAKSDIGEPVSPLDSSVFDFMKRVVLENIKRKEQLMQTIHQNS
AGKRRLPLVRFKASDMSTRMVLVEPEDWAIEDENEKPLVSRVQLPLMLAW
SLSIHKSQGQTLPKVKVDLRRVFEKGQAYVALSRAVSREGLQVLNFDRTR
IKAHQKVIDFYLTLSSAESAYKQLEADE

3D structure

• PDB: 5o6b Insights into the structural and mechanistic basis of multifunctional S. cerevisiae Pif1p helicase.
• Chain: A
• Resolution: 2.029 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.12: DNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T290 G291 T301 H303 S304 G309 L310 V385 K387 T464 R465 N526 N533 S703 H705 K706 F723	T55 G56 T66 H68 S69 G74 L75 V150 K152 T225 R226 N287 N294 S453 H455 K456 F473
BS02	ADP	A	G236 Q241 G261 T262 G263 K264 S265 I266 F416 R417	G1 Q6 G26 T27 G28 K29 S30 I31 F177 R178
BS03	ALF	A	A260 G261 K264 E342 R417 G709 R734	A25 G26 K29 E107 R178 G459 R484

Gene Ontology

Molecular Function

• GO:0003678 DNA helicase activity

Biological Process

• GO:0000723 telomere maintenance
• GO:0006281 DNA repair

External links

• PDB: RCSB:5o6b, PDBe:5o6b, PDBj:5o6b
• PDBsum: 5o6b
• PubMed: 29202194
• UniProt: P07271|PIF1_YEAST ATP-dependent DNA helicase PIF1 (Gene Name=PIF1)