Structure of PDB 5nw7 Chain A

Receptor sequence

>5nw7A (length=440) Species: 5476 (Candida albicans)

SSEKLFRIQCGYQNYDWGKIGSSSAVAQFVHNSDPSITIDETKPYAELWM
GTHPSVPSKAIDLNNQTLRDLVTAKPQEYLGESIITKFGSSKELPFLFKV
LSIEKVLSIQAHPDKKLGAQLHAADPKNYPDDNHKPEMAIAVTDFEGFCG
FKPLDQLAKTLATVPELNEIIGQELVDEFISGIKLPAEVGSQDDVNNRKL
LQKVFGKLMNTDDDVIKQQTAKLLERTDREPQVFKDIDSRLPELIQRLNK
QFPNDIGLFCGCLLLNHVGLNKGEAMFLQAKDPHAYISGDIIECMAASDN
VVRAGFTPKFKDVKNLVEMLTYSYESVEKQKMPLQEFPRSKGDAVKSVLY
DPPIAEFSVLQTIFDKSKGGKQVIEGLNGPSIVIATNGKGTIQITGDDST
KQKIDTGYVFFVAPGSSIELTADSANQDQDFTTYRAFVEA

3D structure

• PDB: 5nw7 Crystal structure of phosphomannose isomerase from Candida albicans complexed with 5-phospho-d-arabinonhydrazide.
• Chain: A
• Resolution: 1.85 Å

Enzymatic activity

• Enzyme Commision number: 5.3.1.8: mannose-6-phosphate isomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	Q111 H113 E138 H285	Q110 H112 E137 H284
BS02	9C2	A	W18 E48 K100 L108 S109 Q111 H113 K136 E138 H285 Y287 E294 K310	W17 E47 K99 L107 S108 Q110 H112 K135 E137 H284 Y286 E293 K309	MOAD: Ki=1.7uM PDBbind-CN: -logKd/Ki=5.77,Ki=1.7uM

Gene Ontology

Molecular Function

• GO:0004476 mannose-6-phosphate isomerase activity
• GO:0008270 zinc ion binding
• GO:0016853 isomerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0000032 cell wall mannoprotein biosynthetic process
• GO:0005975 carbohydrate metabolic process
• GO:0006486 protein glycosylation
• GO:0009298 GDP-mannose biosynthetic process
• GO:0031505 fungal-type cell wall organization

Cellular Component

• GO:0005575 cellular_component
• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:5nw7, PDBe:5nw7, PDBj:5nw7
• PDBsum: 5nw7
• PubMed: 29687459
• UniProt: P34948|MPI_CANAL Mannose-6-phosphate isomerase (Gene Name=PMI1)