Structure of PDB 5n9f Chain A

Receptor sequence

>5n9fA (length=859) Species: 7227 (Drosophila melanogaster)

IRLGCNVSAPSGVLERVKELMEDYSRAPDAKFQQQFRHLLSVNFEEFVAE
TKERNADLDWVNPKLDERLQLELGQRQLEENAKKRLEARKKLPTMKYADD
IIQAVRENQVILIVGSTGCGKTTQVPQILLDDAISRGCASSCRIICTQPR
RISAIAIAEWVSYERCESLGNSVGYQIRLESRKARERASITYCTTGVLLQ
QLQSDPLMHNLSVLILDEIHERSVETDLLMGLLKVILPHRPDLKVILMSA
TVREQDFCDYFNNCPMFRIEGVMFPVKMLYLEDVLSKTNYEFQKPPERRM
KHEAMIEPYLRRIRNSYDSRVLDKLRLPESEGCEDIDFIADLVYYICENE
PEGAILVFLPGYDKISQLYNILDKPKTSKGQRWRDHMAVFPLHSLMQSGE
QQAVFRRPPAGQRKVIISTIIAETSVTIDDVVYVINSGRTKATNYDIETN
IQSLDEVWVTKANTQQRRGRAGRVRPGICYNLFSRAREDRMDDIPTPEIL
RSKLESIILSLKLLHIDDPYRFLQTLINAPNPEAIKMGVELLKRIEALDQ
TGTLTPLGMHLAKLPIDPQMGKMILMSALFCCLDPITSAAAALSFKSPFY
SPLGKESRVDEIKRRMARNMRSDHLMVHNTIIAYRDSRYSHAERDFCYKN
FLSSMTLQQLERMKNQFSELLYNYKFLASSNCKDAASNKNSEKIPLLRAI
IGAGLYPNMAHLRKSRQIKNRVRAIHTMATDDGRRVNFHPSSVNSGESGF
DSAYFVYFQRQKSTDLFLLDSTMVFPMALIIFGDGVEAGVTQNTPYLCVA
KTYYFKCNRETADVVIQLRSNLEKLLLKKALYPAPIEENGYEKQLIKAIE
LLLSLDERL

3D structure

• PDB: 5n9f Structural and mechanistic insights into DHX36-mediated innate immunity and G-quadruplex unfolding
• Chain: A
• Resolution: 2.969 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R211 R212 R239 T255 G257 V258 Q261 Q262 S265 G431 Y432 H463 S464 L465 T489 I490 I491 T494 S495 K511 T513 L524 P635 I636 D637 S664 D680 R793 H809 P810 S811 S833	R150 R151 R178 T194 G196 V197 Q200 Q201 S204 G361 Y362 H393 S394 L395 T419 I420 I421 T424 S425 K441 T443 L454 P565 I566 D567 S594 D610 R723 H739 P740 S741 S763

Gene Ontology

Molecular Function

• GO:0002151 G-quadruplex RNA binding
• GO:0003676 nucleic acid binding
• GO:0003678 DNA helicase activity
• GO:0003723 RNA binding
• GO:0003724 RNA helicase activity
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0051880 G-quadruplex DNA binding

Biological Process

• GO:0032508 DNA duplex unwinding
• GO:0044806 G-quadruplex DNA unwinding

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm

External links

• PDB: RCSB:5n9f, PDBe:5n9f, PDBj:5n9f
• PDBsum: 5n9f
• UniProt: Q8SWT2