Structure of PDB 5mxu Chain A

Receptor sequence
>5mxuA (length=553) Species: 69488 (Penicillium simplicissimum) [Search protein sequence]
EFRPLTLPPKLSLSDFNEFIQDIIRIVGSENVEVISSKIVDGSYMKPTHT
HDPHHVMDQDYFLASAIVAPRNVADVQSIVGLANKFSFPLWPISIGRNSG
YGGAAPRVSGSVVLDMGKNMNRVLEVNVEGAYCVVEPGVTYHDLHNYLEA
NNLRDKLWLDVPDLGGGSVLGNAVERGVGYTPYGDHWMMHSGMEVVLANG
ELLRTGMGALPDPKRPETMGLKPEDQPWSKIAHLFPYGFGPYIDGLFSQS
NMGIVTKIGIWLMPNPRGYQSYLITLPKDGDLKQAVDIIRPLRLGMALQN
VPTIRHILLDAAVLGDKRSYSSRTEPLSDEELDKIAKQLNLGRWNFYGAL
YGPEPIRRVLWETIKDAFSAIPGVKFYFPEDTPENSVLRVRDKTMQGIPT
YDELKWIDWLPNGAHLFFSPIAKVSGEDAMMQYAVTKKRCQEAGLDFIGT
FTVGMREMHHIVCIVFNKKDLIQKRKVQWLMRTLIDDCAANGWGEFRTHL
AFMDQIMETYNWNNSSFLRFNEVLKNAVDPNGIIAPGKSGVWPSQYSHVT
WKL
3D structure
PDB5mxu Two tyrosine residues, Tyr-108 and Tyr-503, are responsible for the deprotonation of phenolic substrates in vanillyl-alcohol oxidase.
ChainA
Resolution2.8 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Y108 D170 E182 D192 D317 V397 E410 I414 H422 H466 F503 R504 K545
Catalytic site (residue number reindexed from 1) Y101 D163 E175 D185 D310 V390 E403 I407 H415 H459 F496 R497 K538
Enzyme Commision number 1.1.3.38: vanillyl-alcohol oxidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD A P99 S101 I102 G103 R104 N105 P169 D170 L171 G174 S175 N179 E182 V185 Y187 V262 W413 H422 F424 R504 K545 P92 S94 I95 G96 R97 N98 P162 D163 L164 G167 S168 N172 E175 V178 Y180 V255 W406 H415 F417 R497 K538
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004458 D-lactate dehydrogenase (cytochrome) activity
GO:0008720 D-lactate dehydrogenase activity
GO:0016491 oxidoreductase activity
GO:0018465 vanillyl-alcohol oxidase activity
GO:0050660 flavin adenine dinucleotide binding
GO:0071949 FAD binding
Biological Process
GO:0015945 methanol metabolic process
GO:1903457 lactate catabolic process
Cellular Component
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005777 peroxisome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5mxu, PDBe:5mxu, PDBj:5mxu
PDBsum5mxu
PubMed28717004
UniProtP56216|VAOX_PENSI Vanillyl-alcohol oxidase (Gene Name=VAOA)

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