Structure of PDB 5mi6 Chain A

Receptor sequence
>5mi6A (length=688) Species: 226186 (Bacteroides thetaiotaomicron VPI-5482) [Search protein sequence]
SLQPPPQQLIVQNKTIDLPAVYQLNGGEEANPHAVKVLKELLSGKQSSKK
GMLISIGEKGDKSVRKYSRQIPDHKEGYYLSVNEKEIVLAGNDERGTYYA
LQTFAQLLKDGKLPEVEIKDYPSVRYRGVVEGFYGTPWSHQARLSQLKFY
GKNKMNTYIYGPKDDPYHSAPNWRLPYPDKEAAQLQELVAVANENEVDFV
WAIHPGQDIKWNKEDRDLLLAKFEKMYQLGVRSFAVFFDDISGEGTNPQK
QAELLNYIDEKFAQVKPDINQLVMCPTEYNKSWSNPNGNYLTTLGDKLNP
SIQIMWTGDRVISDITRDGISWINERIKRPAYIWWNFPVSDYVRDHLLLG
PVYGNDTTIAKEMSGFVTNPMEHAESSKIAIYSVASYAWNPAKYDTWQTW
KDAIRTILPSAAEELESFAMHNSDLGPNGHGYRREESMDIQPAAERFLKA
FKEGKNYDKADFETLQYTFERMKESADILLMNTENKPLIVEITPWVHQFK
LTAEMGEEVLKMVEGRNESYFLRKYNHVKALQQQMFYIDQTSNQNPCQPG
VKTATRVIKPLIDRTFATVVKFFNQKFNAHLDATTDYMPHKMISNVKNLP
LQVKANRVLISPANEVVKWAAGNSVEIELDAIYPGENIQINFEISTDGKE
WKTVDLKQKERLSAGLQKAPVKFVRFTNVSQFVLTIEK
3D structure
PDB5mi6 Increase of enzyme activity through specific covalent modification with fragments.
ChainA
Resolution2.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.2.1.169: protein O-GlcNAcase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NHT A G135 K166 D242 C278 Y282 W337 D344 G132 K163 D239 C275 Y279 W334 D341 PDBbind-CN: -logKd/Ki=7.30,Kd=50nM
BS02 7NQ A Y137 D344 R347 C550 Y134 D341 R344 C547
BS03 CA A E32 E61 D64 E29 E58 D61
Gene Ontology
Molecular Function
GO:0015929 hexosaminidase activity
GO:0016231 beta-N-acetylglucosaminidase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0042802 identical protein binding
GO:0102571 [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine/L-threonine O-N-acetyl-alpha-D-glucosaminase activity
Biological Process
GO:0005975 carbohydrate metabolic process
GO:0006517 protein deglycosylation
GO:1901135 carbohydrate derivative metabolic process

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Molecular Function
View graph for
Biological Process
External links
PDB RCSB:5mi6, PDBe:5mi6, PDBj:5mi6
PDBsum5mi6
PubMed29163914
UniProtQ89ZI2|OGA_BACTN O-GlcNAcase BT_4395 (Gene Name=BT_4395)

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