Structure of PDB 5mi5 Chain A

Receptor sequence
>5mi5A (length=677) Species: 226186 (Bacteroides thetaiotaomicron VPI-5482) [Search protein sequence]
SLQPPPQQLIVQNKTIDLPAVYQLNGGEEANPHAVKVLKELLSGKQSSKK
GMLISIGEKGDKSVRKYSRQIPDHKEGYYLSVNEKEIVLAGNDERGTYYA
LQTFAQLLKDGKLPEVEIKDYPSVRYRGVVEGFYGTPWSHQARLSQLKFY
GKNKMNTYIYGPKDDPYHSAPNWRLPYPDKEAAQLQELVAVANENEVDFV
WAIHPGQDIKWNKEDRDLLLAKFEKMYQLGVRSFAVFFDDISGEGTNPQK
QAELLNYIDEKFAQVKPDINQLVMCPTEYNKSWSNPNGNYLTTLGDKLNP
SIQIMWTGDRVISDITRDGISWINERIKRPAYIWWNFPVSDYVRDHLLLG
PVYGNDTTIAKEMSGFVTNPMEHAESSKIAIYSVASYAWNPAKYDTWQTW
KDAIRTILPSAAEELESFAMHNSDLGPNGHGYRREESMDIQPAAERFLKA
FKEGKNYDKADFETLQYTFERMKESADILLMNTENKPLIVEITPWVHQFK
LTAEMGEEVLKMVEGRNESYFLRKYNHVKALQQQMFYIDQTSNQNPCQPG
VKTATRVIKPLIDRTFATVVKFFNQKFNAHLDATTDYMPHKMKNLPLQVK
ANRVLISPAGNSEIELDAIYPGENIQINFLEISTDGKEWKTVDLKQKSRL
SAGLQKAPVKFVRFTVSDRQFVLTIEK
3D structure
PDB5mi5 Increase of enzyme activity through specific covalent modification with fragments.
ChainA
Resolution2.15 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.2.1.169: protein O-GlcNAcase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 OAN A G135 K166 D242 D243 Y282 W286 V314 N339 D344 G132 K163 D239 D240 Y279 W283 V311 N336 D341 PDBbind-CN: -logKd/Ki=6.77,Kd=170nM
BS02 7NQ A Y137 D344 P549 C550 Y134 D341 P546 C547
BS03 CA A E32 E61 D64 E29 E58 D61
Gene Ontology
Molecular Function
GO:0015929 hexosaminidase activity
GO:0016231 beta-N-acetylglucosaminidase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
GO:0042802 identical protein binding
GO:0102571 [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine/L-threonine O-N-acetyl-alpha-D-glucosaminase activity
Biological Process
GO:0005975 carbohydrate metabolic process
GO:0006517 protein deglycosylation
GO:1901135 carbohydrate derivative metabolic process

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Molecular Function
View graph for
Biological Process
External links
PDB RCSB:5mi5, PDBe:5mi5, PDBj:5mi5
PDBsum5mi5
PubMed29163914
UniProtQ89ZI2|OGA_BACTN O-GlcNAcase BT_4395 (Gene Name=BT_4395)

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