Structure of PDB 5mex Chain A

Receptor sequence
>5mexA (length=322) Species: 3702 (Arabidopsis thaliana) [Search protein sequence]
ESTEFEKNQKRYQDLISTFPHEKGWRPKEPLIEYGGYWWLPSLLEGCIHA
QEFFQARPSDFLVCSYPKTGTTWLKALTFAIANRSRFDDSSNPLLKRNPH
EFVPYIEIDFPFFPEVDVLKDKGNTLFSTHIPYELLPDSVVKSGCKMVYI
WREPKDTFISMWTFLHKERTELGPVSNLEESFDMFCRGLSGYGPYLNHIL
AYWKAYQENPDRILFLKYETMRADPLPYVKSLAEFMGHGFTAEEEEKGVV
EKVVNLCSFETLKNLEANKGEKDREDRPGVYANSAYFRKGKVGDWSNYLT
PEMAARIDGLMEEKFKGTGLLE
3D structure
PDB5mex Structural and biochemical studies of sulphotransferase 18 from Arabidopsis thaliana explain its substrate specificity and reaction mechanism.
ChainA
Resolution1.92 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) K93 H155 S185
Catalytic site (residue number reindexed from 1) K68 H130 S160
Enzyme Commision number 2.8.2.38: aliphatic desulfoglucosinolate sulfotransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PAP A K93 G95 T96 T97 W98 R177 S185 Y243 F284 Y311 F312 R313 K314 K68 G70 T71 T72 W73 R152 S160 Y218 F259 Y286 F287 R288 K289
BS02 SZZ A E54 L65 P92 Y130 I133 H155 F189 L190 E193 Y306 E29 L40 P67 Y105 I108 H130 F164 L165 E168 Y281
BS03 BU2 A R51 P199 R26 P174
Gene Ontology
Molecular Function
GO:0008146 sulfotransferase activity
GO:0016740 transferase activity
GO:0047364 desulfoglucosinolate sulfotransferase activity
GO:0080066 3-methylthiopropyl-desulfoglucosinolate sulfotransferase activity
GO:0080067 4-methylthiobutyl-desulfoglucosinolate sulfotransferase activity
GO:0080068 5-methylthiopentyl-desulfoglucosinolate sulfotransferase activity
GO:0080069 7-methylthioheptyl-desulfoglucosinolate sulfotransferase activity
GO:0080070 8-methylthiooctyl-desulfoglucosinolate sulfotransferase activity
GO:0080071 indol-3-yl-methyl-desulfoglucosinolate sulfotransferase activity
Biological Process
GO:0019761 glucosinolate biosynthetic process
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5mex, PDBe:5mex, PDBj:5mex
PDBsum5mex
PubMed28646214
UniProtQ9C9C9|SOT18_ARATH Cytosolic sulfotransferase 18 (Gene Name=SOT18)

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