Structure of PDB 5ma2 Chain A

Receptor sequence

>5ma2A (length=445) Species: 66821 (Sulfurospirillum multivorans)

AEKEKNAAEIRQQFAMTAGSPIIVNDKLERYAEVRTAFTHPTSFFKPNYK
GEVKPWFLSAYDEKVRQIENGENGPKMKAKNVGEARAGRALEAAGWTLDI
NYGNIYPNRFFMLWSGETMTNTQLWAPVGLDRRPPDTTDPVELTNYVKFA
ARMAGADLVGVARLNRNWVYSEAVTIPADVPYEQSLHKEIEKPIVFKDVP
LPIETDDELIIPNTCENVIVAGIAMNREMMQTAPNSMACATTAFCYSRMC
MFDMWLCQFIRYMGYYAIPSCNGVGQSVAFAVEAGLGQASRMGACITPEF
GPNVRLTKVFTNMPLVPDKPIDFGVTEFCETCKKCARECPSKAITEGPRT
FEGRSIHNQSGKLQWQNDYNKCLGYWPESGGYCGVCVAVCPFTKGNIWIH
DGVEWLIDNTRFLNITEVWDGKINTYGLDADHFRDTVSFRKDRVK

3D structure

• PDB: 5ma2 Cobamide-mediated enzymatic reductive dehalogenation via long-range electron transfer.
• Chain: A
• Resolution: 1.879 Å

Enzymatic activity

• Enzyme Commision number: 1.21.99.5: tetrachloroethene reductive dehalogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	A	R291 M292 I296 C329 C332 K333 C335 C390 P391 F392	R291 M292 I296 C329 C332 K333 C335 C390 P391 F392
BS02	SF4	A	C339 P340 S341 I344 C372 C383 G384 C386	C339 P340 S341 I344 C372 C383 G384 C386
BS03	BVQ	A	Y31 T36 A37 F38 Y170 Y246 M249 N272 Q276 S277 V278 A289 M292 G293 A294 C295 V304 R305 L306 H357 N358 Q359 K362 Q364 Y369 C372 W376 Y382	Y31 T36 A37 F38 Y170 Y246 M249 N272 Q276 S277 V278 A289 M292 G293 A294 C295 V304 R305 L306 H357 N358 Q359 K362 Q364 Y369 C372 W376 Y382
BS04	IOL	A	F38 W56 W96 Y102 Y246 W376 Y382	F38 W56 W96 Y102 Y246 W376 Y382
BS05	IOL	A	L124 P127	L124 P127

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane

External links

• PDB: RCSB:5ma2, PDBe:5ma2, PDBj:5ma2
• PDBsum: 5ma2
• PubMed: 28671181
• UniProt: O68252|PCEA_SULMU Tetrachloroethene reductive dehalogenase (Gene Name=pceA)