Structure of PDB 5lpa Chain A

Receptor sequence
>5lpaA (length=267) Species: 90371 (Salmonella enterica subsp. enterica serovar Typhimurium) [Search protein sequence]
TLSIPPSIQCQTEAACRLITRVTGDTLRAIHLYGSAVAGGLKPNSDIDLL
VTICQPLTEAQRATLMQELLALSSPPGASAEKRALQVTVVLYSQLVPWCF
PPSREMQFGEWLREDICQGIYEPAQQDWDMVLLITQILETSIPLKGERAE
RLFTPAPAAQLLKALRYPLDLWQSTADVQGDEYHIVLTLARIWYTLSTGR
FTSKDAAADWLLPQLPEDYAATLRAAQREYLGLEQQDWHILLPAVVRFVD
FAKAHIPTQFTGHHHHH
3D structure
PDB5lpa Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase fromSalmonella enterica.
ChainA
Resolution1.4 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.47: streptomycin 3''-adenylyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ATP A S36 S46 D49 D130 L133 Q137 R192 I193 T196 K205 Y231 S35 S45 D48 D129 L132 Q136 R191 I192 T195 K204 Y230 MOAD: Kd=50uM
BS02 71R A Q87 W112 W173 D178 D182 H185 Q86 W111 W172 D177 D181 H184 MOAD: Kd=11uM
BS03 CA A D47 D49 D46 D48
BS04 CA A D47 D49 Q87 D46 D48 Q86
Gene Ontology
Molecular Function
GO:0005524 ATP binding
GO:0009012 aminoglycoside 3''-adenylyltransferase activity
GO:0016779 nucleotidyltransferase activity
GO:0046872 metal ion binding
GO:0070566 adenylyltransferase activity
Biological Process
GO:0046677 response to antibiotic

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Molecular Function
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Biological Process
External links
PDB RCSB:5lpa, PDBe:5lpa, PDBj:5lpa
PDBsum5lpa
PubMed29871922
UniProtQ8ZPX9|S3AD_SALTY Aminoglycoside (3'') (9) adenylyltransferase (Gene Name=aadA)

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