Structure of PDB 5lc8 Chain A

Receptor sequence
>5lc8A (length=656) Species: 287 (Pseudomonas aeruginosa) [Search protein sequence]
SIFFSPLKYLGAEQQRSIDASRSLLDNLIPPSLPQYDNLAGKLARRAVLT
SKKLVYVWTENFANVKGVPMARSVPLGELPNVDWLLKTAGVIVELIVNFV
ASLPASAAAQFERIAAGLSGDLEAARQVHEALLEEAKNDPAAAGSLLLRF
TELQTRVIALLTRVGLLVDDILKSASNLGLNRFRAVFGTLRLPEVADSFR
DDEAFAYWRVAGPNPLLIRRVDALPANFPLGEEQFRRVMGADDSLLEAAA
SRRLYLLDYAELGKLAPSGAVDKLLTGTGFAYAPIALFALGKDRAGLLPV
AIQCGQDPATHPMFVRPAESESDLYWGWQMAKTVVQVAEENYHEMFVHLA
QTHLVSEAFCLATQRTLAPSHPLHVLLAPHFEGTLFINEGGARILLPSAG
FIDVMFAAPIQDTQATAGGNRLGFDFYRGMLPESLKARNVDDPAALPDYP
YRDDGLLVWNAIRQWAADYVAVYYASDGDVTADVELAAWVGEVIGSGKVA
GFRPITGRSQLVEVLTMVIFTASAQHAAVNFPQPSMMTYAPAICAMSAAP
APDSPSGKSEADWLKMMPPTLVALEKVNIYHLLGSVYHGRLGDYRQTGFP
YAPVFSDRRVTASGGPLERFQARLKEVEATIRTRNQARRKPYEYLLPSRI
PASTNI
3D structure
PDB5lc8 The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
ChainA
Resolution1.8 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) H377 H382 H555 N559 I685
Catalytic site (residue number reindexed from 1) H348 H353 H526 N530 I656
Enzyme Commision number 1.13.11.12: linoleate 13S-lipoxygenase.
1.13.11.58: linoleate 9S-lipoxygenase.
1.13.11.77: oleate 10S-lipoxygenase.
Interaction with ligand
Site
#
Ligand Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01 FE2 A H377 H382 H555 N559 I685 H348 H353 H526 N530 I656
BS02 ZPE A L182 D190 H377 H382 F415 I416 G420 I423 L424 L161 D169 H348 H353 F386 I387 G391 I394 L395
Gene Ontology
Molecular Function
GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0046872 metal ion binding
Biological Process
GO:0034440 lipid oxidation

View graph for
Molecular Function

View graph for
Biological Process
External links
PDB RCSB:5lc8, PDBe:5lc8, PDBj:5lc8
PDBsum5lc8
PubMed28093240
UniProtQ8RNT4|LOX_PSEAI Linoleate 9/13-lipoxygenase (Gene Name=lox)

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