Structure of PDB 5l6i Chain A

Receptor sequence
>5l6iA (length=1007) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
AAGEIDESLYSRQLYVLGKEAMLKMQTSNVLILGLKGLGVEIAKNVVLAG
VKSMTVFDPEPVQLADLSTQFFLTEKDIGQKRGDVTRAKLAELNAYVPVN
VLDSLDDVTQLSQFQVVVATDTVSLEDKVKINEFCHSSGIRFISSETRGL
FGNTFVDLGDEFTVLDPTGEEPRTGMVSDIEPDGTVTMLDDNRHGLEDGN
FVRFSEVEGLDKLNDGTLFKVEVLGPFAFRIGSVKEYGEYKKGGIFTEVK
VPRKISFKSLKQQLSNPEFVFSDFAKFDRAAQLHLGFQALHQFAVRHNGE
LPRTMNDEDANELIKLVTDLSVQQPEVLGEGVDVNEDLIKELSYQARGDI
PGVVAFFGGLVAQEVLKACSGKFTPLKQFMYFDSLESLPDPKNFPRNEKT
TQPVNSRYDNQIAVFGLDFQKKIANSKVFLVGSGAIGCEMLKNWALLGLG
SGSDGYIVVTDNDSIEKSNLNRQFLFRPKDVGKNKSEVAAEAVCAMNPDL
KGKINAKIDKVGPETEEIFNDSFWESLDFVTNALDNVDARTYVDRRCVFY
RKPLLESGTLGTKGNTQVIIPRLTESYSSSRDPPEKSIPLCTLRSFPNKI
DHTIAWAKSLFQGYFTDSAENVNMYLTQPNFVEQTLKQSGDVKGVLESIS
DSLSSKPHNFEDCIKWARLEFEKKFNHDIKQLLFNFPKDAKTSNGEPFWS
GAKRAPTPLEFDIYNNDHFHFVVAGASLRAYNYGIKSDDSNSKPNVDEYK
SVIDHMIIPEFTPNANLKIQVNDDDPDPDEIDQLVSSLPDPSTLAGFKLE
PVDFEKDDDTNHHIEFITACSNCRAQNYFIETADRQKTKFIAGRIIPAIA
TTTSLVTGLVNLELYKLIDNKTDIEQYKNGFVNLALPFFGFSEPIASPKG
EYNNKKYDKIWDRFDIKGDIKLSDLIEHFEKDEGLEITMLSYGVSLLYAS
FFPPKKLKERLNLPITQLVKLVTKKDIPAHVSTMILEICADDKEGEDVEV
PFITIHL
3D structure
PDB5l6i Dissecting the Specificity of Adenosyl Sulfamate Inhibitors Targeting the Ubiquitin-Activating Enzyme.
ChainA
Resolution2.76 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R21 R481 D544 C600 T601 R603 N781 D782
Catalytic site (residue number reindexed from 1) R12 R472 D535 C591 T592 R594 N772 D773
Enzyme Commision number 6.2.1.45: E1 ubiquitin-activating enzyme.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 B39 A D470 N471 D472 K494 K519 V520 A542 L543 D544 A548 D461 N462 D463 K485 K510 V511 A533 L534 D535 A539 PDBbind-CN: -logKd/Ki=4.35,IC50=45uM
Gene Ontology
Molecular Function
GO:0004839 ubiquitin activating enzyme activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008641 ubiquitin-like modifier activating enzyme activity
GO:0016874 ligase activity
Biological Process
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0006974 DNA damage response
GO:0016567 protein ubiquitination
GO:0036211 protein modification process
GO:0043412 macromolecule modification
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5l6i, PDBe:5l6i, PDBj:5l6i
PDBsum5l6i
PubMed28578874
UniProtP22515|UBA1_YEAST Ubiquitin-activating enzyme E1 1 (Gene Name=UBA1)

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