Structure of PDB 5l1t Chain A

Receptor sequence
>5l1tA (length=397) Species: 29301 (Streptomyces arenae) [Search protein sequence]
TDLPRLPFDNPDIMGIAPQMLALQKEGPIARVGTAGEDAWLVTRYDEVRT
LLADRRLRLSNPNPQPSAKSAARAFSVALMAGDDHETEPARHAQMRSLLI
PRFSTRRLRLMKTRIEHHVDELLDQLAASAPPVDLHRVLSFRLPTMVVCD
LLGVPLADRERFGQWARGTFDQSDNEHSANTFQQVVDYMLELVARKRVEP
GDDILSELIAEKDGALSDADIAHLGNAVLLFGYETTIVRIDLGTLLLLRN
PVQRAQLAEDPGLAPAAVEEILRLGVGGKGSNALIPRYAHGDITVGETVI
RTGDAVMLAIGAANYDDRAFPDGGLFDLTRVRPRSHLAFGHGARHCIGRT
LARIELTAVFERLFRRLPDLRLAVPEESLRWQEHRITGGFDEIPVTF
3D structure
PDB5l1t The Cytochrome P450-Catalyzed Oxidative Rearrangement in the Final Step of Pentalenolactone Biosynthesis: Substrate Structure Determines Mechanism.
ChainA
Resolution2.082 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D172 F232 E235 T236 T237 S282 C347 I348 G349 E356 T388
Catalytic site (residue number reindexed from 1) D171 F231 E234 T235 T236 S281 C346 I347 G348 E355 T387
Enzyme Commision number 1.14.19.8: pentalenolactone synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 7PF A R74 F232 T236 R240 N283 I387 T388 R73 F231 T235 R239 N282 I386 T387 MOAD: Kd=115uM
BS02 HEM A M81 H93 R97 F232 G233 T236 R240 I286 R288 A339 F340 R345 C347 G349 M80 H92 R96 F231 G232 T235 R239 I285 R287 A338 F339 R344 C346 G348
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0016636 oxidoreductase activity, acting on the CH-CH group of donors, iron-sulfur protein as acceptor
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037 heme binding
GO:0046872 metal ion binding
Biological Process
GO:0017000 antibiotic biosynthetic process
GO:1901780 pentalenolactone biosynthetic process

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:5l1t, PDBe:5l1t, PDBj:5l1t
PDBsum5l1t
PubMed27588339
UniProtE3VWI3|PNTM_STRAE Pentalenolactone synthase (Gene Name=pntM)

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