Structure of PDB 5kt4 Chain A

Receptor sequence

>5kt4A (length=378) Species: 9606 (Homo sapiens)

SSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEAGKLGV
KKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGF
DENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLV
GSQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPES
CQHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGI
SVAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELL
ASLLNRVCQDGRKPHTVRLIIRRYGRESRQCPIPSHVIQKLGYDVMTPMV
DILMKLFRNMVNVKFHLTLLSVCFCNLK

3D structure

• PDB: 5kt4 Kinetic and Structural Impact of Metal Ions and Genetic Variations on Human DNA Polymerase iota.
• Chain: A
• Resolution: 2.78 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Q84 K85 Y86 N105 V106 R107 E122 L124 Q325 S328 E329 E330 S332	Q34 K35 Y36 N55 V56 R57 E72 L74 Q275 S278 E279 E280 S282
BS02	dna	A	E152 K232 G266 I267 G268 T271 E383 S384 R385	E102 K182 G216 I217 G218 T221 E327 S328 R329
BS03	0KX	A	D59 L60 C62 F63 Y64 V89 T90 K102 D151	D9 L10 C12 F13 Y14 V39 T40 K52 D101
BS04	MG	A	D59 L60 D151	D9 L10 D101

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:5kt4, PDBe:5kt4, PDBj:5kt4
• PDBsum: 5kt4
• PubMed: 27555320
• UniProt: Q9UNA4|POLI_HUMAN DNA polymerase iota (Gene Name=POLI)