Structure of PDB 5kk7 Chain A

Receptor sequence

>5kk7A (length=455) Species: 9606 (Homo sapiens)

HLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLTLIDA
LDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIRILGGLLSAYH
LSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDST
VAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFIN
THSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGV
RTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPAS
HMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHN
LLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINNV
QDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNAEAHPLP
IWTPA

3D structure

• PDB: 5kk7 Substrate recognition and catalysis by GH47 alpha-mannosidases involved in Asn-linked glycan maturation in the mammalian secretory pathway.
• Chain: A
• Resolution: 1.7324 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): E330 R334 D463 E599
• Catalytic site (residue number reindexed from 1): E86 R90 D219 E355
• Enzyme Commision number: 3.2.1.113: mannosyl-oligosaccharide 1,2-alpha-mannosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	Z5L	A	F329 E397 R461 D463 L525	F85 E153 R217 D219 L281
BS02	MAN	A	R597 E599 F659 E663 A688 E689	R353 E355 F415 E419 A444 E445
BS03	1PS	A	R265 W269 E309 W313 K317	R21 W25 E65 W69 K73
BS04	1PS	A	D255 R632	D11 R388

Gene Ontology

Molecular Function

• GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
• GO:0005509 calcium ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:5kk7, PDBe:5kk7, PDBj:5kk7
• PDBsum: 5kk7
• PubMed: 27856750
• UniProt: Q9UKM7|MA1B1_HUMAN Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (Gene Name=MAN1B1)