Structure of PDB 5kij Chain A

Receptor sequence
>5kijA (length=452) Species: 9606 (Homo sapiens) [Search protein sequence]
HLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLTLIDA
LDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIRILGGLLSAYH
LSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDST
VAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFIN
THSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGV
RTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPAS
HMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHN
LLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINNV
QDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLP
IW
3D structure
PDB5kij Substrate recognition and catalysis by GH47 alpha-mannosidases involved in Asn-linked glycan maturation in the mammalian secretory pathway.
ChainA
Resolution1.649 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) E330 R334 D463 E599
Catalytic site (residue number reindexed from 1) E86 R90 D219 E355
Enzyme Commision number 3.2.1.113: mannosyl-oligosaccharide 1,2-alpha-mannosidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MAN A F329 E397 R461 D463 F85 E153 R217 D219
BS02 MAN A R597 E599 F659 E663 T688 E689 R353 E355 F415 E419 T444 E445
BS03 MAN A D523 L525 E570 D279 L281 E326
BS04 MAN A N327 S375 D376 N83 S131 D132
BS05 1PS A R265 W269 E309 W313 K317 R21 W25 E65 W69 K73
Gene Ontology
Molecular Function
GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
GO:0005509 calcium ion binding
Biological Process
GO:0005975 carbohydrate metabolic process
Cellular Component
GO:0016020 membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5kij, PDBe:5kij, PDBj:5kij
PDBsum5kij
PubMed27856750
UniProtQ9UKM7|MA1B1_HUMAN Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (Gene Name=MAN1B1)

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